By PHAEDON FESSAS F EW EXAMPLES of erythrocytic inclusions due to abnormalities of the hemoglobin molecule are known. The first concerns Hb H.”2 Although the precipitates of Hgb H, such as is observed in the usual test employing brilliant cresyl blue, are produced artificially, there is evidence that some are formed spontaneously in vivo.’4 The second example is that of Hb Zurich; the precipita...

The rate of penetration of brilliant cresyl blue into the living cells of Nitella indicates that the dye enters only in the form of the undissociated molecule. At equilibrium the total concentration of the dye in the sap is proportional to the concentration of the free base in the outside solution.

By PHAEDON FESSAS F EW EXAMPLES of erythrocytic inclusions due to abnormalities of the hemoglobin molecule are known. The first concerns Hb H.”2 Although the precipitates of Hgb H, such as is observed in the usual test employing brilliant cresyl blue, are produced artificially, there is evidence that some are formed spontaneously in vivo.’4 The second example is that of Hb Zurich; the precipita...

A glass electrode apparatus is described with which pH measurements can be made with as small volumes as 2 drops (about 0.14 cc.) of solution. Using this apparatus the change of pH of the vacuolar sap of Nitella, due to the penetration of brilliant cresyl blue, has been readily followed. The sap and the dye have been found to poison the usual type of hydrogen electrode.

The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in γ-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with γ-cyclodextrin.All the evidences indicated that the binding modes between γ-cyclode...