Mammalian cells respond to DNA damage by producing polymers of ADP-ribose (ADPR) on certain DNA-binding proteins (1). Synthesis of variably-sized ADPR polymers correlates with modified protein function (2). Here we present a high resolution electrophoresis technique for separating and quantifying picomole quantities of polymers up to 60 residues long. This technique allows one to study the role...

tinely isolate 80-100 yg of RNA per 8 mL of culture. RNA isolated by this method or with TRIzol (manufacturer's recommended procedure) exhibited an A260-280 ratio of 1.6-1.8. When needed, RNA samples were supplemented with RNA loading dye [0.72 mL formamide, 0.16 mL lox MOPS buffer, 0.26 mL 37% formaldehyde, 0.18 mL distilled water (DEPC-treated), 0.10 mL 80% glycerol, and 8.0 mg bromophenol bl...

The main objective of this work is to evaluate the occurrence of bromophenols (2bromophenol, 4bromophenol, 2,4dibromophenol, 2,6dibromophenol and 2,4,6tribromophenol), in the flesh and guts in two species of the LutjanidaeFamily: Lutjanus synagris and Ocyurus chrysurus. The bromophenols were extracted by steam distillation with pentaneether (7:3 v/v), identified by reverse phase High Performanc...

A series of bromophenol derivatives containing indolin-2-one moiety were designed and evaluated that for their anticancer activities against A549, Bel7402, HepG2, HeLa and HCT116 cancer cell lines using MTT assay in vitro. Among them, seven compounds (4g-4i, 5h, 6d, 7a, 7b) showed potent activity against the tested five human cancer cell lines. Wound-healing assay demonstrated that compound 4g ...

In attempts to develop quantitative techniques for the analysis of serum proteins separated by electrophoresis on filter paper, many workers have estimated the amount of dye taken up by the different protein fractions after staining. WVhen comparisons have been made with analysis by the classical technique, quantitative differences have been noted (Cremer & Tiselius, 1950; Kunkel & Tiselius, 19...

PURPOSE To evaluate and compare the effects of the following dyes on human pigmented epithelial cells: indocyanine green (ICG), infracyanine green (IfCG), trypan blue (TB), bromophenol blue (BrB), patent blue (PB), and Brilliant Blue G (BBG). METHODS ARPE-19 cells cultured in vitro were exposed to these dyes, and acute and chronic toxicity were evaluated. Cell viability was measured by colori...

The present study deals with the adsorption of Bromophenol blue (BB) and Erichrome black T (EBT) – two anionic dyes – onto bentonite carbon composite material (BCC). Batch studies were performed to evaluate the influence of various experimental parameters such as: pH, contact time, adsorbent dose and initial dye concentration. The optimal conditions for the dyes removal were found to be at pH =...

OBJECTIVES The purpose of this study was to investigate the permeation of the low-volatile solvent limonene through different disposable, unlined, unsupported, nitrile exam whole gloves (blue, purple, sterling, and lavender, from Kimberly-Clark). METHODS This study utilized a moving and static dextrous robot hand as part of a novel dynamic permeation system that allowed sampling at specific t...

Epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -PAS-, diastase-PAS; alcian blue pH 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated...

Areas of preneoplastic livers characterized by intense RNA staining apparently represent the sites of neoplastic transformation. These hyperbasophilic foci, as well as the hepatomas, showed a marked decrease in the stain ing intensity of the cytoplasmic proteins in sections stained by the mercury-bromophenol blue method or the MillÃ3nreaction. Following extraction of the RNA, how ever, protein ...