–SDS – Polyacrylamide Gel Electrophoresis (SDS PAGE) of blood plasma from three cattle and one buffalo affected with various tumors (odontoma, nasal granuloma and horn cancer in cattle and teat tumor in buffalo) revealed the presence of new proteins p51, p53 and p71-72 in all the samples which were not present in plasma from normal healthy bovines. However, another protein, p77.5 present in nor...

MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay is commonly used to validate the viability of metabolically active cells. The study was conducted to examine and validate the MTT test to assess the sperm viability of Nili-Ravi buffalo bulls and compare the efficiency of the test with the supra-vital staining technique (eosin-nigrosine) and hypo-osmotic swelling test. F...

The purpose of this study was to determine effects of feeding canola and soybean products as protein supplements on fatty acid composition of adipose tissue and muscle of slaughter bulls and steers and on fatty acid composition of kidney and liver total lipids of bulls. Products included canola meal (CM), extruded canola (EC, full-fat), ground canola (GC, full-fat), soybean meal (SBM), and extr...

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which measures the reduction of MTT, is commonly used to validate the viability of metabolically active cells. This study was conducted to evaluate and validate the MTT assay to assess the spermatozoal viability of Nili-Ravi buffalo bulls and compare the efficiency of the test with the supravital staining technique (e...

Dimensional characteristics and proportions of live spermatozoa in permanent nigrosin\p=m-\eosinpreparations from the semen samples of Murrah buffalo bulls were studied in different seasons. Between bulls, all the characteristics varied significantly. Between seasons, all the characteristics, except length of head and mid-piece, varied significantly. Environmental temperature and humidity both ...

Integrative management of wildlife and livestock requires a clear understanding of the diseases transmitted between the two populations. The tick-borne protozoan parasite Theileria parva causes two distinct diseases in cattle, East Coast fever and Corridor disease, following infection with parasites derived from cattle or buffalo, respectively. In this study, cattle were immunized with a live s...

Theileria buffeli/orientalis is a group of benign and mildly pathogenic species of cattle and buffalo in various parts of the world. In a previous study, we identified T. buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe-iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterise the 18S...

Three groups of young buffalo in captivity were infected by exposing them to similar buffalo in the acute stages of infection induced by needle inoculation with SAT 1 or 2 viruses. Clear foot lesions developed in most of the buffalo from which the relevant virus types were re-isolated. During the first week following infection virus was found in blood, nasal secretions, saliva, preputial secret...

Aim The aim of present study was to determine seroprevalence of bluetongue virus (BTV) in Haryana state of India. Materials and Methods A total of 803 serum samples, 408 of cattle and 395 of buffalo origin, respectively, were collected from different villages of Haryana. Sampling was done randomly to obtain unbiased results. The samples were evaluated by a competitive enzyme-linked immunosorb...

A serological survey of brucellosis in cattle and buffalo was performed in 23 States of India. A total of 30,437 bovine samples, comprising 23,284 cattle and 7,153 buffalo (Bubalus bubalis), were screened. The screening initially used the rose bengal plate test: doubtful and positive samples were then titrated in the serum tube agglutination test. The overall prevalence rate of antibodies was 1...