Mapping of resistance genes to Puccinia polysora in maize The objectives of this study were to identify microsatellite markers linked to resistance gene of resistance to Puccinia polysora and to estimate the phenotypic effect of these genes on two monocyclic variables lesion, number and length. Two inbreed lines with different degrees of resistance (Z-95 and Z-93), the hybrid (Z-95 X Z-93) and ...

The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP marker...

Identification of candidate genomic regions associated with target traits using conventional mapping methods is challenging and time-consuming. In recent years, a number of single nucleotide polymorphism (SNP)-based mapping approaches have been developed and used for identification of candidate/putative genomic regions. However, in the majority of these studies, insertion-deletion (Indel) were ...

A yellow seed coat is a desirable agronomic trait in the seeds of oilseed-type Brassica crops. In this study, we identified a candidate gene for seed coat color in Dahuang, a landrace of Brassica rapa. A previous study of Dahuang mapped the seed coat color gene Brsc1 to a 2.8-Mb interval on chromosome A9 of B. rapa. In the present study, the density of the linkage map for Brsc1 was increased by...

Cucumber is an agriculturally and economically important vegetable crop worldwide. Fruit flesh thickness is an important trait for cucumber and also a central determinant of yield, yet little is known about the underlying mechanism of this trait. In this study, bulked segregant analysis (BSA) combined with specific length amplified fragment sequencing (SLAF-seq) was applied to finely map the ge...

Oryza meyeriana is highly resistant to rice bacterial blight (BB) and this resistance trait has been transferred to cultivated rice (O. sativa) using asymmetric somatic hybridization. However, no resistance genes have yet been cloned. In the present study, a progeny of the somatic hybridization with high BB resistance was crossed with a rice cultivar with high BB susceptibility to develop an F2...

We used a next-generation high-throughput sequencing platform to resequence the Xinguowei and Shouxing melon cultivars, the parents of Fengwei melon. We found 84% of the reads (under a coverage rate of "13×") placed on the reference genome DHL92. There were 2,550,000 single-nucleotide polymorphisms and 140,000 structural variations in the two genomes. We also identified 1,290 polymorphic genes ...

Introduction Multiple nodes and dwarf mutants in barley are a valuable resource for identifying genes that control shoot branching, vegetative growth development. Methods In this study, physiological, microscopic genetic analysis were conducted to characterize fine-map the underling gene of mutant with Stem Nodes Spikes Dwarf ( msnsd ), which was selected from EMS- 60 Co-treated cv . Edamai 934...

Peel color is one of the most important appearance qualities eggplant. The main pigment in peel green-fruited eggplant chlorophyll, while white-fruited a mutant chlorophyll biosynthesis. A dominant gene Gv controls biosynthesis peel, but none its genes have been mapped. In this study, white-peel inbred line 19141, green-peel 19143, and their F2 progeny with 3:1 segregation ratio plants to plant...