In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...

The aim of our research was to examine the ability of density gradient preparation BoviPure and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activity, membrane integrity and acrosomal status were evaluated and compared befor...

The objective of this study was to validate the assessment of bull sperm morphology done by veterinary practitioners. Out of 1606 bulls, 1400 (87.2%) and 1344 (83.7%) were designated by practitioners and an experienced andrologist, respectively, as having > 70% morphologically normal sperm. In 92% of the evaluations, there was agreement between the designations chosen.

Oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. Cadmium (Cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. Because of its long biological half-life (10–30 years), Cdaccumulates in the biological systems. The present study was designed to assess the concen...

This review reexamines parameters needed for optimization of flow cytometric sexing mammalian sperm and updates the current status of sperm sexing for various species where this technology is currently being applied. Differences in DNA content have provided both a method to differentiate between these sex-determining gametes and a method to sort them that can be used for predetermining sex in m...

The methylation status of sperm DNA differs between individual bulls. However, the relationship between methylation status and bull sperm parameters is not well elucidated. The present study investigated genome-wide methylation profiles at 450,000 CpG sites in bull spermatozoa by using a human DNA methylation microarray. Semen samples from three adult Japanese Black bulls with different in vitr...

There are two populations of sperm in bull semen. One consists of X-chromosome-bearing sperm; the other consists of Y-chromosome-bearing sperm. In contrast, all cattle ova contain an X chromosome. That is why when an X-chromosome-bearing sperm fertilizes the ovum a female (XX) develops, and when a Y-chromosome-bearing sperm fertilizes the ovum, a male (XY) develops. Ratios of the number of male...

THE sperm tail-stump defect has been reported in Holstein bulls in Canada (Coubrough and Barker 1964) and Denmark (Blom 1976), and also in Charolais (Williams 1987), Ayrshire (Vierula and others 1983, 1987, Arriola and others 1985, Foote and others 1992) and polled Hereford (Peet and Mullins 1991) bulls. This short communication describes a case in a Holstein bull, which was investigated by usi...

There are many procedures available for evaluating semen, and except for culling obviously inferior samples, none of the procedures is highly correlated with fertility. The most commonly used method is estimating sperm motility subjectively, and this method is appropriate for culling substandard semen; this approach also can be used for more stringent quality control and for experiments if done...