BACKGROUND Complement activation during reperfusion of ischemic myocardium augments myocardial injury, and complement inhibition with C1-esterase inhibitor (C1-INH) at the time of reperfusion exerts marked cardioprotective effects in experimental studies. Application of C1-INH in newborns, however, was recently reported to have dangerous and even lethal side effects. This study addresses the es...

We present a new functional assay for the first complement component (C1) in plasma, based on its activation by inhibition of the C1-esterase inhibitor (C1-inh) when monospecific antiserum to C1-inh is added to the plasma. After maximal activation, we can determine the concentration of activated C1 by using an amidolytic rate assay with a chromogenic substrate. We have optimized the assay condi...

We have produced transgenic mice expressing human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements. The transgene was generated using a minigene construct in which most of the human C1 inhibitor gene (C1NH) was replaced by C1 inhibitor cDNA. The construct retained the promoter region extending 1.18 kb upstream of the transcription start site, introns...

Craniovertebral junction surgery requires knowledge regarding the anatomy of this region, particularly the C1 vertebra. Both C1 laminectomy and C1-2 instrumentation necessitate preoperative information about bony landmarks and the vertebral artery. This study compares the results obtained from anatomic and computed tomographic measurements of C1 bony landmarks. 31 C1 cervical vertebrae were mea...

Definition. A couple of complex reduced projective plane curves C1 and C2 of a same degree is said to make a Zariski pair, if there exist tubular neighborhoods T (Ci) ⊂ P of Ci for i = 1, 2 such that (T (C1), C1) and (T (C2), C2) are diffeomorphic, while the pairs (P, C1) and (P , C2) are not homeomorphic; that is, the singularities of C1 and C2 are topologically equivalent, but the embeddings ...

SALSA MLPA probemix P027 Uveal melanoma Page 1 of 7 SALSA MLPA probemix P027-C1 Uveal melanoma Lot C1-0814 & C1-0213 & C1-0211: A large number of probes have been replaced by other probes in the same chromosomal regions as compared to previous lots, and several reference probes have been replaced/included. Details are in Table 1. As a result, the version number has been changed to C1. In additi...

Ric c1, an allergenic protein from Ricinus communis , is an insect α-amylase inhibitor that has become an occupational allergen. Ric c1 can cross-react with allergens from wheat, soybean, peanut, shrimp, fish, gluten, house dust, tobacco, and air fungus, thereby amplifying the concern and risks caused by Ricinus allergens. Two continuous IgE-binding epitopes were identified in Ric c1, both cont...

Carbon isotope fractionation factors associated with the aerobic consumption of methane (C1), ethane (C2), propane (C3), and nbutane (C4) were determined from incubations of marine sediment collected from the Coal Oil Point hydrocarbon seep field, located offshore Santa Barbara, CA. Hydrogen isotope fractionation factors for C1, C2 and C3 were determined concurrently. Fresh sediment samples fro...

The synthesis of the subunits of the C1 complex (C1q, C1s, C1r), and its regulator C1 inhibitor (C1-Inh) by human monocytes has been previously established. However, surface expression of these molecules by monocytes has not been shown. Using flow cytometry and antigen-capture enzyme-linked immunosorbent assay, we show here for the first time that, in addition to C1q, peripheral blood monocytes...

Human C1-esterase inhibitor (C1-INH) is a unique anti-inflammatory multifunctional plasma protein best known for its key role in regulation of the classical complement pathway, contact activation system and intrinsic pathway of coagulation. By sequence homology and mechanism of protease inhibition it belongs to the serine proteinase inhibitor (serpin) superfamily. However, in addition to its in...