Recent evidence points to homeotic proteins as actors in the crosstalk between development and DNA replication. The present work demonstrates that HOXC13, previously identified as a new member of human DNA replicative complexes, is a stable component of early replicating chromatin in living cells: it displays a slow nuclear dynamics due to its anchoring to the DNA minor groove via the arginine-...

Resistance to chemotherapy is a primary problem for the effective treatment of ovarian cancer. Recently, increasing evidence has demonstrated that miRNAs modulate many important molecular pathways involved in chemotherapy. Previous studies demonstrated that miR-199a affected ovarian cancer cell resistance to cisplatin (DDP). However, the role of miR-199a and its target genes in determination of...

The difficulties in detection of C13 magnetic resonance signals in organic compounds which arise from the low natural abundance and the small magnetic moment of C'3 are well known.' For complex substances of biochemical interest, further reduction in signal-to-noise ratio occurs because of extensive carbonproton spin-spin interactions. Considerable relief from these difficulties is possible in ...

The current study aimed to monitor organic pollution on island and coastal environments using linear alkylbenzene (LAB). aquatic environment is affected by the hazardous chemicals discharged through domestic industrial waste. distribution, composition, sources of LABs in sediments Port Dickson coast Pulau Merambong were identified gas chromatography–mass spectrometry (GC–MS). Chains ranging fro...

The retinal protonated Schiff base of bacteriorhodopsin is photoreactive to reducing agents such as NaBH4. In the present work we have studied the effect of different protein hydration levels on the photoreductive reaction, as well as the consequences of preventing isomerization around the critical C13=C14 retinal double bond. It was revealed that the rate of light-induced NaBH4 reaction can be...

Aims: The aims were to test whether Parvibaculum lavamentivorans degraded commercial linear alkylbenzenesulphonate (LAS) surfactant via x-oxygenation and b-oxidation to sulphophenylcarboxylates (SPCs), whether the organism was widespread and reisolable, and whether the degradative community used the 4-sulphocatechol 1,2-dioxygenase to cleave the aromatic ring from LAS. Methods and Results: Hete...

BHK 21 (C13) cells inoculated with a wild strain of Leptospira interrogans serovar Pomona isolated from a porcine abortion exhibited cythopathic effect (CPE) consistent in degenerative morphological alterations whereas no cell changes or alterations were observed in cells infected with a non-pathogenic Leptospira biflexa serovar Patoc strain. BHK 21 (C13) cells were seeded on 24 well cell cultu...

Density functional theory (DFT) calculations based on the self-consistent-charge tight-binding approximation have been performed to study the influence of the protein pocket on the 3-dimensional structure of the 11-cis-retinal Schiff base (SB) chromophore. Starting with an effectively planar chromophore embedded in a protein pocket consisting of the 27 next-nearest amino acids, the relaxed chro...

The transcriptional regulation of myeloperoxidase (MPO) and lactoferrin (LF) was examined during terminal myeloid differentiation of the murine cell line 32D C13. The rates of transcription initiation for MPO and LF, determined by an in vitro nuclear run-on assay, increased approximately ninefold. The accumulation of MPO mRNA in 32D C13 cells, determined by Northern blot analysis, correlated te...