background : the development of chemoresistance represents a major obstacle in the successful treatment of cancers such as non-hodgkin’s lymphoma (nhl). with the recognition of important roles for both p53 and its more recently described paralog p73 in mediating the activity of anti-cancer drugs, there has been increasing recognition that cellular resistance to anthracyclines could and does a...

BHK 21 (C13) cells inoculated with a wild strain of Leptospira interrogans serovar Pomona isolated from a porcine abortion exhibited cythopathic effect (CPE) consistent in degenerative morphological alterations whereas no cell changes or alterations were observed in cells infected with a non-pathogenic Leptospira biflexa serovar Patoc strain. BHK 21 (C13) cells were seeded on 24 well cell cultu...

The effect of the tyrosine kinase inhibitor genistein on the accumulation of cisplatin (DDP) was investigated in DDP-sensitive and -resistant human 2008 ovarian cardnoma cell lines. DDP accumulation after a 1-h exposure was maximally increased by concurrent 40 piM genistein. The maximal stimulation of accumulation was observed after 2 h of total genistein exposure and was 83 ± 13% (n = 5) highe...

Low-passage BHK21/C13 cells respond to cytochalasin B (CB) by undergoing limited or controlled nuclear division. These cells respond to CB as normal cells do since nuclear division usually occurs only once. Premature chromosome condensation, a result of mitoses in highly multinucleate cells, occurs in less than 0.5% of the cells. When they are inoculated into weanling hamsters, s. c. tumors app...

background: adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines.  it has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer.  this study has managed to investigate endogenous and/or exogenous expression of visfatin and resisti...

background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...

many cell lines, when cultured under proper conditions, can form three dimensionalstructures called multicellular spheroids. these spheroids resemble in vivo tumor models in severalaspects. therefore, studying growth characteristics and behavior of spheroids is beneficial inunderstanding the behavior of tumors under various experimental conditions. in this work, we havestudied the growth proper...

An efficient strategy for the construction of C13-oxidized cembrenolides is reported. Central to this strategy is the installation of the C13 hydroxyl group prior to cembrane macrocyclization (via formation of the C1-C2 bond), allowing access to both C13 alcohol epimers. The orientation of the C13 alcohol was found to influence the cyclization mode of the cembranolide scaffold upon furan oxidat...

Resistance to chemotherapy is a primary problem for the effective treatment of ovarian cancer. Recently, increasing evidence has demonstrated that miRNAs modulate many important molecular pathways involved in chemotherapy. Previous studies demonstrated that miR-199a affected ovarian cancer cell resistance to cisplatin (DDP). However, the role of miR-199a and its target genes in determination of...

The effect of the tyrosine kinase inhibitor genistein on the accumulation of cisplatin (DDP) was investigated in DDP-sensitive and -resistant human 2008 ovarian carcinoma cell lines. DDP accumulation after a 1-h exposure was maximally increased by concurrent 40 micrometer genistein. The maximal stimulation of accumulation was observed after 2 h of total genistein exposure and was 83 +/- 13% (n ...