A rapid, sensitive and reproducible HPLC method using amperometric detector was developed and validated for the analysis of clarithromycin in human plasma. The separation was achieved on a monolithic silica column (MZ- C8 125×4.0 mm) using acetonitrile- methanol-potassium dihydrogen phosphate buffer (40:6:54,v/v), with pH of 7.5, as the mobile phase at a flow rate of 1.5 mL/min. The assay enabl...

A rapid, sensitive and reproducible HPLC method using amperometric detector was developed and validated for the analysis of clarithromycin in human plasma. The separation was achieved on a monolithic silica column (MZ- C8 125×4.0 mm) using acetonitrile- methanol-potassium dihydrogen phosphate buffer (40:6:54,v/v), with pH of 7.5, as the mobile phase at a flow rate of 1.5 mL/min. The assay enabl...

gc-fid and hplc-dad were developed for determination of menadione (mn) and menadione sodium bisulphite (msb). by means of each method, quantitative analysis of msb in commercial pharmaceutical was performed in both direct analysis of msb and analysis of mn by converting msb to mn with sodium carbonate. gc-fid method was carried out on the hp-5 capillary column using nitrogen gas. hplc-dad metho...

A nanosecond time-resolved resonance Raman (ns-TR3) spectroscopic study of the reactions of the 2-fluorenylnitrenium ion with several C8-substituted guanosine derivatives is reported. The TR3 spectra show that the 2-fluorenylnitrenium ion reacts with the C8-substituted guanosine derivatives (C8-methylguanosine and C8-bromoguanosine) to produce C8 intermediates with the methyl and bromine moieti...

An accurate, precise and reproducible RP-HPLC UV Spectrophotometric method was developed validated for the simultaneous estimation of Metoprolol Telmisartan in tablet dosage form. The chromatographic separation carried out on X-tera C8 column (100mm*4.6mm*5µ), by using mobile phase (0.05M Sodium phosphate buffer pH 2.8 methanol) ratio 35:65, at a flow rate 1.2ml/min. detection wave length 226nm...

Aromatic amines are bioactivated to electrophilic compounds that react with DNA, predominantly at the C8 position of guanine bases. This site is weakly nucleophilic and it has been proposed that the C8 adduct is the final product after initial N7-adduct formation. To consider this possibility, we reacted several C8-substituted guanine derivatives with N-acetoxy-2-aminofluorene, prepared in situ...

The internalization of a basic peptide, 001-C8 [H-MeTyr-Arg-MeArg-d-Leu-NH(CH2)8NH2], into enterocyte-like Caco-2 cells was evaluated. Internalization of125I-labeled 001-C8 (125I-001-C8) increased time dependently and reached steady state at 60 min. The steady-state internalization of 125I-001-C8 (7.24 ± 0.41 μl/mg protein) was temperature and concentration dependent and was significantly decre...

We have developed an on-line solid-phase extraction (SPE) method coupled to high-performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) for measuring trace levels of 18 perfluorinated chemicals (3 perfluorosulfonates, 8 perfluorocarboxylates, 7 perfluorosulfonamides) in serum. Without protein precipitation, only dilution with 0.1 M formic acid, one aliquot of 100 microL of se...