ERGIC-53 and VIP36 are categorized as leguminous type (L-type) lectins, and they function as cargo receptors for trafficking certain N-linked glycoproteins in the secretory pathway in animal cells. They share structural similarities in their carbohydrate recognition domains (CRDs) but exhibit distinct sugar-binding specificities and affinities. VIP36 specifically interacts with the α1,2-linked ...

Combined deficiency of factor V and factor VIII (F5F8D) is a bleeding disorder caused by mutations in either LMAN1 or MCFD2. LMAN1 (ERGIC-53) and MCFD2 form a Ca(2+)-dependent cargo receptor that cycles between the endoplasmic reticulum (ER) and the ER-Golgi intermediate compartment for efficient transport of FV/FVIII from the ER to the Golgi. Here we show that the C-terminal EF-hand domains ar...

The molecular interplay between cargo recognition and regulation of the activity of the kinesin-1 microtubule motor is not well understood. Using the lysosome adaptor SKIP (also known as PLEKHM2) as model cargo, we show that the kinesin heavy chains (KHCs), in addition to the kinesin light chains (KLCs), can recognize tryptophan-acidic-binding determinants on the cargo when presented in the con...

Mutations in myosin VI have been associated with autosomal-recessive (DFNB37) and autosomal-dominant (DFNA22) deafness in humans. Here, we characterise an myosin VI nonsense mutation (R1166X) that was identified in a family with hereditary hearing loss in Pakistan. This mutation leads to the deletion of the C-terminal 120 amino acids of the myosin VI cargo-binding domain, which includes the WWY...

Single-span mobile pendulum-type ropeways formed by two coupled self-propelled wheeled chassis of high load capacity and cross-country capability are a promising type transport reloading equipment under difficult operating conditions. This article discusses the problem assessing weight productivity such various technological options modes their operation. A computational method for determining ...

Synchromodality is a freight transport process in which information exchanged expeditiously order to maximize the benefits of different modes and nodes terms efficiency environmental impact. The aim study analyze problems synchronized intermodal traffic management between main port inland European corridors find reliable solutions these problems. Therefore, purpose this article investigate prob...

This study is part of the project Improved Port/Ship interface (IPSI) , supported by the EU Commission. The general objective of the project is to strengthen the economic position of waterborne transport, especially short sea shipping. In the IPSI-concept, the terminal is the central point of inter modal logistic chains. This paper concerns a simulation study, with the overall objective to prov...

Within the extended receptor cycle of peroxisomal matrix import, the function of the import receptor Pex5p comprises cargo recognition and transport. While the C-terminal half (Pex5p-C) is responsible for PTS1 binding, the contribution of the N-terminal half of Pex5p (Pex5p-N) to the receptor cycle has been less clear. Here we demonstrate, using different techniques, that in Saccharomyces cerev...