Helicobacter pylori produces a metalloprotease with a native molecular size of approximately 200 kDa, as determined by size-exclusion chromatography. Subcellular distribution studies demonstrated that the activity was associated with the outer membrane fraction of the bacterium. In addition, the protease was secreted by the bacterium when grown in liquid culture. The enzyme activity was measure...

PURPOSE Behaviour of the vein thrombus is determined by the activity ratio of coagulation factors to factors of fibrinolytic system. The aim of the study is to evaluate activity of some coagulation and fibrinolytic factors in the vein thrombus. MATERIAL AND METHODS The activity of platelets aggregating factors, tissue factor, thrombin, antithrombins, antiheparin factors, plasminogen activator...

The first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; produce a wide variety of extracellular enzymes involved in the degradation protein, chitin, and lipids. main objectives were to assay enzymatic activity Cordyceps locustiphila anamorph, solid liquid medium at different temperatures also evaluate pathogenicity against South American ...

BACKGROUND Aseptic loosening of hip prosthesis as it occurs in clinical cases in human patients was attributed to wear particles of the implants, the response of the tissue dominated by macrophages and the production of inflammatory mediators and matrix degrading enzymes; however, the cascade of events initiating the process and their interaction regarding the time course is still open and disc...

Proteases catalyze the hydrolysis of a peptide bond of a protein; they are widely distributed in nature. They perform various biological functions in plants and particularly in humans they participate in zymogen activation, fibrinolysis, complement activation, blood coagulation and digestion process. Objectives: The present study focused on evaluation of in vitro total proteolytic activity from...

conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

N, N-Dimethyl proteins were prepared by reductive methylation of their amino groups with formaldehyde and NaBH4. Proteolysis of these alkylated proteins by trypsin, or-chymotrypsin, subtilisin, pepsin, and fungal protease was determined by direct measurement of the bonds split, with the use of trinitrobenzenesulfonic acid to determine directly the appearance of new terminal amino groups. The lo...

A simple, fast, reproducible, and reliable fibrometer method for determination of plasma or serum plasminogen was developed. Results obtained with this method were compared to those obtained with a Coseinolytic method and a Chromogenic Substrate S-2251 method. For all three procedures, serum or plasma samples were acidified before the activation process. Conversion of plasminogen to plasmin dur...