Quantitative trait locus (QTL) analysis of genetic crosses has proven to be a useful tool for identifying loci associated with specific phenotypes and for dissecting genetic components of complex traits. Inclusion of a mutation that interacts epistatically with QTLs in genetic crosses is a unique and potentially powerful method of revealing the function of novel genes and pathways. Although we ...

The objective of this study was to examine the effect short term transportation and lairage time on meat quality, myofibrillary structure calpastatin (CAST), µ-calpain (CAPN1), calpain 3 (CAPN3) heat shock protein 70 (HSPA1A) expression levels longissimus dorsi Kıvırcık breed sheep (n=40). Meat quality evaluated by muscle glycogen amounts, pH, temperature, cooking loss, water holding capacity, ...

The aim of the study was to evaluate the association between the microstructure characteristics of longissimus lumborum (LL) muscle and the genotype at porcine loci MSTN and CAST. The study was carried out on 132 unrelated pigs – 93 crosses of Pietrain and (Polish Large White x Polish Landrace) and 39 Stamboek castrated males. Crosses Pi x (PLW x PL), with an equal proportion of castrated males...

1,3-Butadiene (BD) is a common environmental contaminant classified as "carcinogenic to humans." Formation of BD-induced DNA adducts plays a major role in its carcinogenicity. BD is also an epigenotoxic agent (i.e., it affects DNA and histone methylation in the liver). We used a panel of genetically diverse inbred mice (NOD/LtJ, CAST/EiJ, A/J, WSB/EiJ, PWK/PhJ, C57BL/6J, and 129S1/SvImJ) to ass...

Molecular markers for beef tenderness are classic examples of the contribution of genome technology to animal breeding through marker-assisted selection (MAS). Markers on the μ-calpain (CAPN1) and calpastatin (CAST) genes have been extensively evaluated for their association with tenderness. However, little is known about their potential effect on other economically important traits. In this wo...

The objectives of the study were to evaluate allelic frequencies and to test the association of polymorphisms in the calpastatin (CAST) and µ-calpain (CAPN1) genes with meat tenderness in 3 French beef breeds. A total of 1,114 Charolais, 1,254 Limousin, and 981 Blonde d'Aquitaine purebred young bulls were genotyped for 3 SNP in the CAST gene and 4 SNP in the CAPN1 gene. Two of these markers, 1 ...

Relevance and methods . For molecular genetic analysis, samples taken from Romanov sheep in micro-populations of the Yaroslavl region ear by pluck were isolated. Polymorphism genes type 3 protein that inhibits insulin-like growth factor (IGFBP-3), hormone (Gh), calpastatin (CAST) was studied. A selection assessment live weight indicators age periods, depending on genotype, carried out. Results ...

The hallmark of age-related (presbycusis) and noise-induced hearing loss is high-frequency (> 20 kHz) hearing loss. Through a collaborative study with TMGC (Tennessee Mouse Genome Consortium), seventeen ENU-induced mouse mutation strains with high-frequency hearing loss have been identified, but affected genes are yet identified. As a first step in identifying the gene/s underlying the ENU muta...

Investigation was carried out on 96 [(Polish Large White × Polish Landrace) × (Duroc × Pietrain)] fatteners. The results concerning the influence CAST × RYR1 genes interaction on some meat quality parametres suggest that incidence of faulty, exudative meat appearing in some stress-resistant fatteners (CC genotype in relation to RYR1 locus) and also normal meat in some stress-sensitive fatteners...