In the course of long-term infection with Porphyromonas gingivalis in adult periodontitis, a specific antibody response to this organism is generated. We describe a potential novel approach for identifying an immunodominant antigen in human periodontitis patients. First, various monoclonal antibodies (MAbs) were established from mice immunized with crude antigen preparations of P. gingivalis FD...

Lipopolysaccharide O antigens (endotoxins) and other bacterial antigens readily attach to erythrocytes in vitro. This attachment is prevented by certain mammalian and avian sera. In this study, the inhibitory capacity of sera from lower animals was compared with that of higher animals for a total of 30 species. Antigens and the corresponding antisera included both crude O antigens and purified ...

Plant diseases caused by viruses are the most menace to sustainable agriculture, leading numerous billion dollars in losses annually. Proper and accurate detection of plant is always key developing appropriate solutions manage economic them. Advances technology have simplified tools available detect diagnose at a time when control measures should be used. Currently, nucleic acid serological bas...

Submit Manuscript | http://medcraveonline.com Abbreviations: 95%CI: 95% Confidence Interval; ANC: Antenatal Care Attendee; aOR: Adjusted Odds; Ratio; cOR: Crude Odds Ratio; HBV: Hepatitis B Virus; HBeAg: Hepatitis B e antigen; HBsAg: Hepatitis B Surface Antigen; Anti-HBc: Hepatitis B Core Antibody; Anti-HBe: Hepatitis Be Antibody; Anti-HB: Hepatitis B Surface Antibody; HIV: Human Immunodeficien...

Submit Manuscript | http://medcraveonline.com Abbreviations: 95%CI: 95% Confidence Interval; ANC: Antenatal Care Attendee; aOR: Adjusted Odds; Ratio; cOR: Crude Odds Ratio; HBV: Hepatitis B Virus; HBeAg: Hepatitis B e antigen; HBsAg: Hepatitis B Surface Antigen; Anti-HBc: Hepatitis B Core Antibody; Anti-HBe: Hepatitis Be Antibody; Anti-HB: Hepatitis B Surface Antibody; HIV: Human Immunodeficien...

background: the aim of this study was to evaluate the possible use of biomphalaria alexandrina snail antigens in diagnosis of schistosomiasis mansoni using enzyme linked immunolectrotransfere blot (eitb). methods: s. mansoni adult worm crude antigens (awa), feet and visceral humps of b. alexandrina and bulinus truncatus were used. hyperimmune mice sera (his) versus each antigen were prepared fo...

A diagnostic antigen, C-antigen, was evaluated in serodiagnosis of alveolar hydatid disease by Western blotting. The C-antigen migrated diffusely to the region of 30-35 kDa in 8% polyacrylamide-SDS gel electrophoresis. We isolated this antigen from a crude metacestode extract by a simple method of phenol/chloroform extraction. The isolated antigen was resistant to boiling, proteolysis and acid ...

BACKGROUND Amoebic liver abscess (ALA) is the most frequent clinical presentation of extra-intestinal amoebiasis. The diagnosis of ALA is typically based on the developing clinical symptoms, characteristic changes on radiological imaging and serology. Numerous serological tests have been introduced for the diagnosis of ALA, either detecting circulating amoebic antigens or antibodies. However th...

Anisakis simplex crude extracts (CE) (IPI, ASAC and ALK-ABELLO), A. simplex larval antigens purified using a column of IgG anti-A. simplex (PAK) or a column of IgG anti-Ascaris suum (PAS), antigen eluted from columns of IgG anti-A. suum (EAS) and an A. suum adult CE were assayed by the skin prick test. Thirty percent of assayed patients showed a negative reaction in the Anisakis skin prick test...