Introduction P-glycoprotein (P-gp) expression on activated lymphocytes in systemic lupus erythematosus (SLE) plays a role in active efflux of intracellular drugs, resulting in drug resistance. The role of P-gp-expressing lymphocytes in the pathogenesis of SLE remains unclear. The aim of this study was to determine the importance of P-gp+CD4+ cells in organ manifestations in refractory SLE. Me...

This study characterizes the early steps of T lymphocyte activation in healthy elderly subjects. The expression of CD69, the earliest inducible antigen which appears with T lymphocyte activation, was assessed in T cells cultured with medium, anti-CD3 or PMA. The proliferative responses of T cells stimulated through CD69 and CD3 pathways were also studied. Donors included 31 healthy elderly [age...

Cell adhesion molecules have a key role in the migration of T cells to inflammatory foci. However, the effect of the endothelial-lymphocyte interaction on the activation of the latter cells remains unresolved. We have studied the effect of resting and stimulated endothelial cells (ECs) on the activation of peripheral blood T cells (PBTLs), as assessed by the expression of CD69 and CD25 activati...

Abstract INTRODUCTION Glioblastoma (GBM) is the most malignant brain tumor in adults, with a dismal prognosis despite aggressive therapy. Immunotherapy currently being evaluated as treatment modality for recurrent GBM. MRI not adequate response assessment to immunotherapy even after using refined criteria. Thus, there need development of neuroimaging techniques assessment. T-cells are key media...

PURPOSE To assess the effects of mycophenolate mofetil (MMF) therapy on T helper cell activation status, using CD69 expression and cytokine profile with flow cytometry in relation to clinical activity in uveitis. METHODS Patients with posterior or intermediate uveitis treated with MMF (n = 10), patients with active uveitis not treated with MMF and receiving no or minimal therapy (n = 10), and...

Although aflatoxins (AFs) have been shown to be immune-suppressive agents in animals, the potential role of AFs in modifying the distribution and function of leukocyte subsets in humans has never been assessed. We examined the cellular immune status of 64 Ghanaians in relation to levels of aflatoxin B1 (AFB1)-albumin adducts in plasma. The percentages of leukocyte immunophenotypes in peripheral...

This study investigated the effect of caffeine on antigen-stimulated lymphocyte activation. Six males rested for 3.5 h after ingesting 0 (PLA), 2, or 6 (6CAF) mg·kg(-1) body mass of caffeine. The number of antigen-stimulated NK CD69(+) cells increased in 6CAF at 1 h compared with PLA (P = 0.021). Caffeine did not influence the number of antigen-stimulated CD69(+) T cells or the geometric mean f...

Lactic acid was originally a by-product of glucose metabolism, but many studies have shown that lactic plays vital role in regulating immune cell polarization, differentiation and effector functions. In this study, we provide evidence low pH impaired the function CD4+ T cells response to TCR non-TCR stimulation. Specifically, or hydrochloric treatment significantly decreased IL-2 IFN-γ producti...

Abstract CD69 depletion from the lymphoid compartment promotes a Th17/Treg imbalance and exacerbates development of atherosclerosis. As atherosclerosis is trigger myocardial infarction, we have analyzed role in Treg cells after a) permanent occlusion left-anterior-descending coronary artery (LAD-ligation) mice, b) angiography two cohorts acute infarction (MI) patients. Our data show that expres...