Diffuse large B-cell lymphoma (DLBCL) can be divided into prognostically important subgroups with germinal center B-cell-like (GCB), activated B-cell-like (ABC), and type 3 gene expression profiles using a cDNA microarray. Tissue microarray (TMA) blocks were created from 152 cases of DLBCL, 142 of which had been successfully evaluated by cDNA microarray (75 GCB, 41 ABC, and 26 type 3). Sections...

This work is concerned with the development of an automatic image processing tool for DNA microarray images. This paper proposes, implements and tests a new tool for cDNA image analysis. The DNAs are imaged as thousands of circularly shaped objects (spots) on the microarray image and the purpose of this tool is to correctly address their location, segment the pixels belonging to spots and extra...

OBJECTIVES This study was designed to identify a common gene expression signature in dilated cardiomyopathy (DCM) across different microarray studies. BACKGROUND Dilated cardiomyopathy is a common cause of heart failure in Western countries. Although gene expression arrays have emerged as a powerful tool for delineating complex disease patterns, differences in platform technology, tissue hete...

We calculate the variances of two classes of estimates of differential gene expression based on log ratios of fluorescence intensities from cDNA microarray experiments: direct estimates, using measurements from the same slide, and indirect estimates, using measurements from different slides. These variances are compared and numerical estimates are obtained from a small experiment involving 4 sl...

April 19, 2009 Version 1.0.2 Date 2007-07-13 Maintainer Deli Wang Author Deli Wang, Jian Huang Depends splines Title A two-way semilinear model for normalization and analysis of cDNA microarray data. Description twslm is for normalization of cDNA microarray data with the two-way semilinear model. Huber’s and Tukey’s bisquare weight functions are available for robust esti...