introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment cas...

Two flocks of ewes were used as sources of embryos for embryo transfer programs: a Merino flock comprising 3 lines differentially selected on weaning weight and a Coopworth flock consisting of three lines differentially selected for backfat thickness. Effects of embryo genotype, year of transfer, sire and number of embryos transferred on embryo survival were examined. No significant line or sir...

Background: Though plausible candidate adhesion systems have been identified, current knowledge of embryo-maternal attachment in human is limited by the inability to conduct well-controlled functional investigations. We have sought a viable medium-throughput model for the identification and functional assessment of molecular markers in the initial epithelial phases of implantation. An ideal mod...

Placental abnormalities occur frequently in cloned animals. Here, we attempted to isolate trophoblast stem (TS) cells from mouse blastocysts produced by somatic cell nuclear transfer (NT) at the blastocyst stage (NT blastocysts). Despite the predicted deficiency of the trophoblast cell lineage, we succeeded in isolating cell colonies with typical morphology of TS cells and cell lines from the N...

BACKGROUND We have previously reported the retrospective observation that when at least one embryo, transferred on day 3, expressed sHLA-G above the geometric mean (sHLA-G+) 46 h post-ICSI, there was a marked improvement in both pregnancy (PR) and implantation (IR) rates. METHODS The media surrounding individual embryos derived from ICSI performed on oocytes from 482 women < or =43 years of a...

Interspecies somatic cell nuclear transfer (iSCNT) involves the transfer of a nucleus or cell from one species into the cytoplasm of an enucleated oocyte from another. Once activated, reconstructed oocytes can be cultured in vitro to blastocyst, the final stage of preimplantation development. However, they often arrest during the early stages of preimplantation development; fail to reprogramme ...

Background Mesenchyme stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and c...

Background: Morphology and presence of Polar body in the oocyte are denoting oocyte quality and is used as a criterion for prognosis of embryo quality. However, little is known about ICSI result of oocytes with two polar bodies. The present study reports the result of ICSI in a case having several oocytes with two polar bodies. Materials and Methods: A 34 years old patient (K.H.) with history o...