A human melanoma was cultivated in tissue culture chambers on coverslips, under strips of dialysis cellophane. I t was found with phase contrast microscopy that cells emigrating from these cultures contained a Golgi complex and forms suggestive of the endoplasmic reticulum. Both of these observations are documented with photographs and discussed with respect to findings in melanomas by electron...

Islet neogenesis-associated protein (INGAP) is a protein expressed during islet neogenesis. We have cloned a novel cDNA having a similar sequence to INGAP cDNA. The cDNA encodes 175 amino acids designated INGAP-related protein (INGAPrP). INGAP is expressed in cellophane-wrapped pancreas, but not in normal pancreas, whereas INGAPrP was abundantly expressed in normal pancreas.

THE correction of electrolyte imbalance and the removal of retention products of the blood during renal failure is at present achieved by the use of artificial kidneys which permit the diffusion of solutes through a semi-permeable membrane. Several types of apparatus used successfully for hemodialysis include those described by Kolff and Watschinger (1956) and Alwall, Norviit, and Steins (1948)...

Embryo chick leg bones were cultivated under sheets of dialysis cellophane in multipurpose culture chambers. The osteoblasts emigrating in this environment contained phase gray juxtanuclear bodies from which phase white droplets emanated. These were concluded to be the Golgi complex and are described with respect to their living morphology, microscopically observed functional activity, vital st...

The binding of T-1824 (Evans blue) with plasma has been extensively studied in the past by Allen and Orahovats (1) by means of the cellophane sorption method. Although this approach yielded excellent results, a simpler and more direct method seemed desirable. The basis for this new method is furnished by the observation, made in 1939,’ that the free T-1824 gives rise to a distinct polarographic...

It has been shown that thin-film dialysis can be performed in such a way that the limiting rate is the rate of entry of the solute into the membrane from the high-concentration side. The rate of diffusion, therefore, reflects the probability of a molecule entering the pores on the surface and does not depend on the resistance to diffusion offered by the internal structure of the membrane. The g...