A review with 90 references is presented to show the development of acidic chemiluminescence methods for biochemical analysis by use of flow injection technique in the last 10 years. A brief discussion of both the chemiluminescence and flow injection technique is given. The proposed methods for biochemical analysis are described and compared according to the used chemiluminescence system.

We describe a method for the detection of gangliosides on thin-layer chromatography plates using enhanced chemiluminescence. In contrast to previously published colorimetric techniques, the use of chemiluminescence of detect antibody binding to gangliosides has increased sensitivity and simplicity. In addition, the use of chemiluminescence lacks the hazards of radioactivity, and produces a perm...

Pasteurella haemolytica A1 crude leukotoxin (25%, vol/vol) rapidly diminished the bovine neutrophil chemiluminescence response to opsonized zymosan. This inhibition was neither prevented nor reversed by 75 mM sucrose. Dilute leukotoxin did not directly stimulate neutrophil chemiluminescence nor did it alter the chemiluminescence response of the neutrophils to opsonized zymosan.

In situ and perfused rat livers showed a spontaneous chemiluminescence of 7-12 counts/sec . cm2 (corresponding to 7-12 x 10(3) photons/sec . cm2); chemiluminescence was increased up to 30 times by infusion of exogenous hydroperoxides. The chemiluminescence of the perfused liver was oxygen dependent. Ethyl, t-butyl, and cumene hydroperoxides were almost equally effective in inducing light emissi...

a sensitive and fast chemiluminescence (cl) method has been described for the direct determination of atropine. the method is based on the quenching effect of atropine on the cl signals of the luminol– h2o2 reaction catalyzed by hemin in an alkaline medium. hemin could strongly enhance the chemiluminescence of the luminol–h2o2 system. the luminophor of the luminol–h2o2–hemin cl system was ident...

Recently it has been shown that the natural killer (NK)-target cell interaction results in an immediate burst of chemiluminescence. In this paper we investigated to apply chemilumines cence assay for the evaluation of human NK cell activities. Degrees of chemiluminescence were dependent on the concentration of lymphocytes or tar. get cells, but optimal concentrations of lymphocytes and target c...

Chemiluminescent acridinium dimethylphenyl ester labels are used in automated immunoassays for clinical diagnostics. Light emission from these labels is triggered by alkaline peroxide in the presence of the cationic surfactant cetyltrimethylammonium chloride (CTAC). The surfactant plays a critical role in the chemiluminescence process of these labels by both accelerating their emission kinetics...

This paper reports a microchip with an integrated passive micromixer based on chaotic advection. The micromixer with staggered herringbone structures was used for luminol-peroxide chemiluminescence detection. The micromixer was examined to assess its suitability for chemiluminescence reaction. The relationship between the flow rate and the location of maximum chemiluminescence intensity was inv...

The spontaneous emission of chemiluminescence appears to reflect changes in the intracellular steady-state concentration of oxygen radicals. Stimulation of arachidonic acid metabolism in human platelets also induces chemiluminescence, which indicates the formation of highly reactive species by cyclooxygenase andlor lipooxygenase [1,2]. It was observed that arachidonic acid, linoleic acid and li...

Blood phagocytes from patients with asthma have an increased capacity to produce reactive oxygen metabolites. We studied whether whole blood luminol-dependent chemiluminescence could detect this phenomenon in patients with a normal spirometry but bronchial hyperreactivity as determined with a methacholine bronchial challenge test. Whole blood chemiluminescence, serum eosinophilic cationic prote...