Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced p...

Background: Human chorionic gonadotropin (hCG) is a member of glycoprotein hormones family consist of two different non-covalently heterodimeric chains: alpha and beta subunits with 92 and 145 amino acids respectively. This hormone plays an important role in human reproduction and physiology especially for maintenance of the corpus luteum during the first months of pregnancy Materials and Metho...

Biopharmaceutical proteins are usually produced by culturing recombinant Chinese hamster ovary (CHO) cells. High producer cell lines screened from transfected cells with random integration of target genes. Since transgene expression is susceptible to the surrounding environment integrated genomic locus, should be selected a large number heterogeneous insertion. In contrast, targeted into charac...

Abstract Transparency is widespread in nature, ranging from transparent insect wings to ocular tissues that enable you read this text, and marine vertebrates. And yet, cells tissue models biology are usually strongly light scattering optically opaque, precluding deep optical microscopy. Here we describe the directed evolution of cultured mammalian toward increased transparency. We find mutation...

Three mammalian cell lines were examined for their ability to metabolize the regulatory oxysterol, 25-hydroxycholesterol, and derepress 3-hydroxy-3-methylglutaryl CoA reductase. In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via side-chain hydroxylation and scission of the C20-C22 bond. Chinese hamster lung cells did not appe...

The human O6-alkylguanine-DNA alkyltransferase complementary DNA was transfected into the alkyltransferase-deficient Chinese hamster ovary cell line, D422, in an effort to dissect the underlying mechanisms of bischloroethylnitrosourea (BCNU)-induced mutations. The alkyltransferase-transformed cell line exhibited 100-fold protection against BCNU-induced toxicity and an overall decrease in mutati...

The effect of 26-hydroxycholesteroI and other intermediates in bile acid synthesis on HMG-CoA reductase activity was studied in Chinese hamster ovary (CHO) cell culture. Incubation of CHO cells for 5 hr in 0.25 PM 26-hydroxycholesterol caused a 40% inhibition of HMG-CoA reductase activity. All other intermediates tested including 3B-hydroxy-5cholenoic acid and cholest-5-ene-3B,7a,26-triol, oxid...

The mutational specificity of the alkylating agent 1-3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) was analyzed at the endogenous hemizygous adenine phosphoribosyl transferase gene of the Chinese hamster ovary cell line D422. A 1-h treatment of the Chinese hamster ovary cells with 50 microM BCNU resulted in a toxicity level of 62% and induced mutation at this target with a frequency of 32.1 mutant...

DNA lesions, cytotoxicity, and cellular uptake of CaCrO4 were com pared in Chinese hamster ovary, in mouse embryo fibroblast C3H10T'/2, and in human osteosarcoma cells. The concentration of CaCrO4 that reduced colony formation by 50% was 2or 3-fold less in human osteo sarcoma cells than in C3H 10T'/2 or Chinese hamster ovary cells. Alkaline elution studies showed that CaCrO4 induced DNA single ...