The cDNA encoding the 326 amino acids of a Class I basic chitinase gene from Leucaena leucocephala de Wit (KB3, Genbank accession: AAM49597) was cloned under the control of CaMV35S promoter in pCAMBIA 1300 and transferred to Koshihikari. Calli of Koshihikari rice was transformed with agrobacterium with this construct expressing the chitinase and βglucouronidase (GUS). The frequencies of calli 9...

Acidic mammalian chitinase (AMCase) is produced in the lung during allergic inflammation and asthma, and inhibition of enzymatic activity has been considered as a therapeutic strategy. However, most chitinase inhibitors are nonselective, additionally inhibiting chitotriosidase activity. Here, we describe bisdionin F, a competitive AMCase inhibitor with 20-fold selectivity for AMCase over chitot...

Many fungi, including pathogenic strains, require proper chitin metabolism to assure normal cell wall replication. Chitinase hydrolyzes chitin; inhibition of endogenous chitinases or application of extracellular chitinases can disrupt fungal division. It is possible that chitinase inhibitors could be used as antifungal agents. We have solved the X-ray structure of a class I1 chitinase from barl...

Chitinases are the hydrolytic enzymes which protect plants against pathogen attack. However, the precise role of chitinases in disease resistance has not been explored in wheat. In the present study, in silico approach, including secondary structure analysis, detailed signature pattern study, cis-acting regulatory elements survey, evolutionary trends and three-dimensional molecular modeling was...

the chitinase producing ability of pseudomonas fluorescens strains viz., pf1, pb2 and fp7 was evaluated in a culture medium with and without a chitin source. the addition of 1% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. among three strains tested, fp7 responded well to the addition of chitin by producing 31.2% increased chitinase in c...

background and aims. class iii malocclusions are considered as one of the most complex orthodontic problems to diagnose and treat. numerous studies have been conducted to determine the morphologic characteristics of craniofacial complex in patients with this malocclusion. the aim of this study was to determine the dentofacial characteristics of class iii malocclusion in mashhadian adults. mater...

statement of problem: due to the appearance of palatal height difference in orthodontic patients we decided to carry out this study. purpose: the purpose of this research was to determine palatal height in persons with normal occlusion and different malocclusions (class i, ii div i and iii) and comparing them with each other. materials and methods: in this cross sectional research, 240 subjects...

Tropical carnivorous plant, Nepenthes, locally known as "monkey cup", utilises its pitcher as a passive trap to capture insects. It then secretes enzymes into the pitcher fluid to digest the insects for nutrients acquisition. However, little is known about the microbiota and their activity in its pitcher fluid. Eighteen bacteria phyla were detected from the metagenome study in the Nepenthes pit...

conclusion a significant relationship exists between the type of facial skeletal classification and the shape of the sella turcica; as in class iii patients, sella turcica bridge was reported with a higher frequency. also, sella turcica had a significantly higher length in these patients than in those with class i and class ii facial skeletal types. results sella turcica had normal morphology i...