In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol-chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol-chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with s...

Accumulating evidence implicates cerebral fat embolism (CFE) as a causative agent in post-operative confusion (POC). CFE occurs following orthopaedic procedures including, intra-medullary (IM) nailing and total joint arthroplasty (TJA). The incidence of CFE is high (59-100% TJA) and the resulting POC is associated with higher overall complication rates. Cognitive dysfunction improves in many pa...

In this paper, a high performance flexible component that serves as a color filter and an electrode simultaneously is suggested. The suggested highly conductive and flexible color filter electrode (CFE) has a multilayer film structure composed of silver (Ag) and tungsten trioxide (WO3). The CFE maintained its color filtering capability even when the films were bent on a polyethylene terephthala...

CFE is the largest power producer and supplier in Mexico with 65,000 active workers. CFE operates and maintains 150 production plants generating more than 37,000 Megawatt-hours of electricity a year. The utility also maintains more than 67,000 kms. of transmission lines serving 21 million customers in the whole country. This paper documents an analysis under the light of Enterprise Application ...

This study reports the effects of long-term elevated atmospheric CO2 on root production and microbial activity, biomass, and diversity in a chaparral ecosystem in southern California. The free air CO2 enrichment (FACE) ring was located in a stand dominated by the woody shrub Adenostoma fasciculatum. Between 1995 and 2003, the FACE ring maintained an average daytime atmospheric CO2 concentration...

Using a pulse chase (13)CO2 plant labeling experiment we compared the flow of plant carbon into macromolecular fractions of rhizosphere soil microorganisms. Time dependent (13)C dilution patterns in microbial cellular fractions were used to calculate their turnover time. The turnover times of microbial biomolecules were found to vary: microbial RNA (19 h) and DNA (30 h) turned over fastest foll...

T he present work presents the extraction of copper(II) from sulphuric acid solutions with Sodium diethyldithiocarbamate (SDDT), and six different organic diluents: Dichloromethane, Chloroform, Carbon tetrachloride, Toluene, xylene and Cyclohexane, were tested. The pair SDDT/Chloroform showed to be the most selective in removing the copper cations, and hence was considered throughout the experi...

This study examines gender differences and laterality in maximal handgrip strength and controlled force exertion (CFE) in young adults. The subjects were 75 healthy young males (mean age 19.6 ± 1.6 yrs.) and 50 healthy young females (mean age 20.9 ± 1.9 yrs.). Maximal handgrip strength was measured twice. The subjects performed the CFE test three times after one practice trial. They matched the...

the traditional extraction method was used to evaluate the antioxidant properties of nettle leaves in soybean oil. also the effects of three different solvents (water, 80% methanol and chloroform) on the extraction content of phenolic and flavonoid compounds from nettle leaves were examined. water extract had the highest extraction yield, and chloroform extract had the highest extraction conten...

Sulfuryl fluoride is a colorless, odorless gas used as fumigant against pests during the storage of durable agricultural products. It also one alternatives to methyl bromide, which banned by Montreal Protocol. During fumigation, decomposition product sulfuryl residue can be formed in addition residue. Fluoride actually natural ingredient tea plant. However, within growing process plant concentr...