Garrido JL and Zapata M (1996) Ion-pair reversed-phase high-performance liquid chromatography of algal chlorophylls. Journal of Chromatography A 738: 285. Hodgson DA, Wright SW and Davies N (1997) Mass spectrometry and reverse phase HPLC techniques for the identiRcation of degraded fossil pigments in lake sediments and their application in palaeolimnology. Journal of Paleolimnology 18: 335. Jef...

The embryos of some angiosperms (usually referred to as chloroembryos) contain chlorophylls during the whole period of embryogenesis. Developing embryos have photochemically active chloroplasts and are able to produce assimilates, further converted in reserve biopolymers, whereas at the late steps of embryogenesis, seeds undergo dehydration, degradation of chlorophylls, transformation of chloro...

BACKGROUND AND AIMS There are several studies suggesting that tomato (Solanum lycopersicum) chromoplasts arise from chloroplasts, but there is still no report showing the fluorescence of both chlorophylls and carotenoids in an intermediate plastid, and no video showing this transition phase. METHODS Pigment fluorescence within individual plastids, isolated from tomato fruit using sucrose grad...

Chloroplast thylakoid membranes isolated in the presence of EDTA retain high rates of O(2) evolution (>/=340 mumol.h(-1).mg chlorophyll(-1)) but contain no Mn(2+) that is detectable by electron paramagnetic resonance (EPR) at room temperature. The total Mn(2+) content of these preparations is 4.6 per 400 chlorophylls; 0.6 Mn(2+) can be released by addition of Ca(2+), a treatment that does not a...

Photosystem I (PSI) is an essential component of photosynthetic membranes. Despite the high sequence and structural homologies, its absorption properties differ substantially in algae, plants and cyanobacteria. In particular it is characterized by the presence of low-energy chlorophylls (red forms), the number and the energy of which vary in different organisms. The PSI-LHCI (PSI-light harvesti...

In a previous paper (l), studies were made of chlorophyll degradation products frequently to be found in leaf extracts. The solation of spectroscopically pure chlorophylls a and b was then considered (2). The logical outcome of these studies is the application of the data to the determination of chlorophyll in extracts. It is complicated by the suggestion that chlorophyll as known to Willstatte...

In a previous paper (l), studies were made of chlorophyll degradation products frequently to be found in leaf extracts. The solation of spectroscopically pure chlorophylls a and b was then considered (2). The logical outcome of these studies is the application of the data to the determination of chlorophyll in extracts. It is complicated by the suggestion that chlorophyll as known to Willstatte...

The. separation and identification of pigments, chlorophylls and carotenoids, of several teas by high-performance liquid chromatography (HPLC) were described. HPLC was carried out using a Symmetry Cs column with a photodiode array detection. Pigments were eluted with a binary gradient of aqueous pyridine solution at a flow rate of 1.0 ml per min at 25°C. HPLC analyses archived the separation of...

Pigments are important contributors to the appearance and healthful properties of both avocado fruits and the oils extracted from these fruits. This study determined carotenoid and chlorophyll pigment concentrations in the skin and three sections of the flesh (outer dark green, middle pale green, and inner yellow flesh-nearest the seed) and anthocyanin concentrations in the skin of Hass avocado...