To compare the sensitivity of various protocols for methicillin-resistant Staphylococcus aureus (MRSA) surveillance, active surveillance for detecting MRSA nasal colonization was performed on 97 members of the medical staff and 218 patients in the Intensive Care Unit (ICU) of a university hospital. Duplicate nasal swabs were collected from each participant. One was plated directly on a blood ag...

A total of 138 isolates, 118 methicillin-resistant Staphylococcus aureus (MRSA) isolates (staphylococcal cassette chromosome type II, 20 isolates, type III, 39 isolates and type IV, 59 isolates) and 20 methicillin-sensitive S. aureus isolates were evaluated by phenotypic methods: cefoxitin and oxacillin disk diffusion (DD), agar dilution (AD), latex agglutination (LA), oxacillin agar screening ...

Methicillin-resistant Staphylococcus aureus (MRSA) accounts for more than 70% of S. aureus isolates from tertiary hospitals in Korea. Clinical isolates of S. aureus were collected from eight provincial, university-affiliated hospitals during the period from June 1999 to January 2001 for nationwide surveillance. All isolates were screened for reduced susceptibility to vancomycin by using brain h...

A novel chromogenic medium, Spectra MRSA (Remel, Lenexa, KS), was designed to detect methicillin-resistant Staphylococcus aureus (MRSA) rapidly and more efficiently than traditional media (i.e., tryptic soy agar with 5% sheep blood [SBA] and mannitol salt agar [MSA]). A multicenter study (including four clinical trial sites and the Medical College of Wisconsin [MCW] Milwaukee, WI) compared the ...

AIM To determine the presence of MRSA in hunting dogs in Maiduguri metropolitan. MATERIALS AND METHODS Phenotypic methods used includes microscopic technique, colony morphology study, catalase-coagulase tests, and the use of mannitol salt agar test, oxacillin resistance screening agar base, and antibiotic susceptibility testing methods. Genotypic approach was used for deoxyribonucleic acid ex...

Introduction The heterogeneous expression of methicillin resistance in Staphylococcus aureus (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, Staphylococcus aureus strains ...

Method Fifty isolates of MRSA from different specimens like blood, urine, exudates and respiratory were collected from 2010 to 2011. MRSA screen slide latex agglutination test kit (Denka Seikan, Japan) was used for PBP2′ detection and Oxacillin Resistant Screen Agar Base (ORSAB) with supplement (Oxoid Ltd., UK) was used for detection of MRSA from the isolates with ATCC controls. The detection o...

Background & Aims: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. The aim of this study was to determine the risk factors of nasal carriage of MRSA and its antibiotic susceptibility pattern among healthcare workers at Namazi Hospital (Shiraz-Iran) Methods: In a cross-sectional study from July to November 2006, nasal swabs were taken from 600 stratif...

This study was carried out to see the methicillin resistance of Staphylococcus aureus isolated from patients with skin and soft tissue infection. In this study, a total of 600 S. aureus isolates isolated from 1,334 specimens from skin and soft tissue infection were included. For the screening of methicillin resistant of S. aureus (MRSA), specimens were inoculated on lipovitellin mannitol salt a...

Abstract 205 isolates of actinomycetes bacteria were isolated from soil samples collected Satun province, Thailand. To have more chances to get diverse groups bacteria, treated using three different methods before inoculated on two selective media, humic acid-vitamin agar and coal vitamin agar. After months incubation at 30°C, all typically characteristic colonies picked purified then tested fo...