Two dimensional thin layer chromatography on polyamide sheets is a useful method for identification of PTH-amino acids which have very close Rf-values. The solvent systems described here are particularly effective and have an edge over other solvent systems used for the separation of PTH-amino acids. The solvent systems provide a clear separation of all PTH-amono acids except lle / Leu in a...

The packed column supercritical fluid chromatography has risen as a promising alternative separation technique to the conventional liquid and gas chromatography. Although many advantages compared other chromatographic techniques, its mechanism is not fully understood due complex combination effects of parameters on quality lacking global strategies for studying mechanisms. This review aims prov...

Class separation of methylated free bile acids from bile acids conjugated with taurine and methylglycine was accomplished using a solvent system of 2,2,4-trimethylpentane-absolute ethanol 10:1 (v/v). By developing a silica thin-layer plate two times with solvent in a Brinkmann sandwich tank, the difficult resolution between methyl cholate and methyl glycolithocholate was achieved. Evidence is p...

Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons fo...

Chromatography is a vital biophysical method that allows for the separation, identification, and filtering of components in order to conduct quantitative research. High efficiency, quicker separation speed, high flow rate, increased detection sensitivity, cost savings are all advantages novel chromatography methods. The goal using chromatography, which technique investigation separated from its...

Se-containing proteins play an important role in human metabolic processes such as antioxidant action. However, speciation analysis of Se-containing proteins in biological samples is challenging because of their small amounts. In order to determine selenoprotein P (Sel-P) in human plasma, separation performance was compared between size exclusion chromatography and affinity chromatography. It w...

The separations of alcohols with hydrophilic and hydrophobic parts, and the separation of aromatic mixtures, are extremely important processes in gas and petroleum industries. Choosing an adsorbent for performing this separation is the most important part of the process. Silica gel is used as an adsorbent is various techniques such as pressure swing adsorption (PSA) and gas and liquid chromatog...

A unidimensional thin-layer chromatography system for the separation of phospholipids has been developed. The method employs precoated plates impregnated with boric acid and is capable of resolving eight phospholipid standards, including phosphatidylinositol, phosphatidylserine, and phosphatidylglycerol. Liver, kidney, and platelet phospholipids of the rat were resolved into six major classes u...

Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that t...

The separation of free fatty acids was performed with supercritical fluid chromatography (SFC) on analytical lab scale apparatus. Experiments were performed using fatty acids, which occur in vegetable oils in different amounts. Free fatty acids were analyzed without derivatization. Several stationary phases of packed columns and types of detectors were used to achieve efficient free fatty acids...