نتایج جستجو برای: chromosomal integration

تعداد نتایج: 268172  

Journal: :PLoS ONE 2008
Alberto Albanese Daniele Arosio Mariaelena Terreni Anna Cereseto

Integration of the double-stranded DNA copy of the HIV-1 genome into host chromosomal DNA is a requirement for efficient viral replication. Integration preferentially occurs within active transcription units, however chromosomal site specificity does not correlate with any strong primary sequence. To investigate whether the nuclear architecture may affect viral integration we have developed an ...

Hassan Motejadded, Josef Altenbuchner

A new system is presented for the generation of recombinant <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt...

2013
Wenli Zhang Martin Muck-Hausl Jichang Wang Chuanbo Sun Maren Gebbing Csaba Miskey Zoltan Ivics Zsuzsanna Izsvak Anja Ehrhardt

We recently developed adenovirus/transposase hybrid-vectors utilizing the previously described hyperactive Sleeping Beauty (SB) transposase HSB5 for somatic integration and we could show stabilized transgene expression in mice and a canine model for hemophilia B. However, the safety profile of these hybrid-vectors with respect to vector dose and genotoxicity remains to be investigated. Herein, ...

Journal: :Metabolic engineering 2016
Shuobo Shi Youyun Liang Mingzi M Zhang Ee Lui Ang Huimin Zhao

Despite recent advances in genome editing capabilities for the model organism Saccharomyces cerevisiae, the chromosomal integration of large biochemical pathways for stable industrial production remains challenging. In this work, we developed a simple platform for high-efficiency, single-step, markerless, multi-copy chromosomal integration of full biochemical pathways in Saccharomyces cerevisia...

Journal: :Journal of virology 2005
Hongjie Wang Dmitry M Shayakhmetov Tobias Leege Michael Harkey Qiliang Li Thalia Papayannopoulou George Stamatoyannopolous André Lieber

Gene therapy for hemoglobinopathies requires efficient gene transfer into hematopoietic stem cells and high-level erythroid-specific gene expression. Toward this goal, we constructed a helper-dependent adenovirus vector carrying the beta-globin locus control region (LCR) to drive green fluorescent protein (GFP) expression, whereby the LCR-GFP cassette is flanked by adeno-associated virus (AAV) ...

2016
Mario Juhas James W. Ajioka

BACKGROUND Escherichia coli K-12 is a frequently used host for a number of synthetic biology and biotechnology applications and chassis for the development of the minimal cell factories. Novel approaches for integrating high molecular weight DNA into the E. coli chromosome would therefore greatly facilitate engineering efforts in this bacterium. RESULTS We developed a reliable and flexible la...

Journal: :Carcinogenesis 2002
Panu Peitsaro Sakari Hietanen Bo Johansson Taina Lakkala Stina Syrjänen

Infection with human papillomavirus (HPV) of specific high-risk type triggers a series of events in target cells, which will eventually lead to development of genital neoplasia. The integration of high-risk HPV DNA into the cell genome has been regarded as a crucial event in tumor progression. With respect to different HPV types, the knowledge of HPV integrated loci is still limited. We have no...

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