Chromosome anomalies belong to genetic factors, which participate on etiology of reproductive failure. The aim of the study was to investigate the frequency of chromosome abnormalities in infertile men in the Prešov region in Slovakia (1998-2014). Karyotyping using G-banding and C-banding methods was performed in 1426 subjects including 948 infertile men and 478 controls. Karyotype analyses rev...

The karyotypes of Luciliacluvia (Walker, 1849) and Luciliasericata (Meigen, 1826) from Argentina were characterized using conventional staining and the C- and G-like banding techniques. Besides, nucleolus organizer regions (NORs) were detected by fluorescent in situ hybridization (FISH) and silver staining technique. The chromosome complement of these species comprises five pairs of autosomes a...

In order to have a good perspective of wild animals, it is necessary to determine their population and genetic structure. It provides an opportunity to decide on better conservation managements. In the wilderness, due to the escapable nature and sometimes not having the distinguishable bisexual appearance, sex identification could be difficult by observing animals. The X- and Y- chromosome link...

the anopheles stephensi mysorensis originating from kazeroon area south of iran, which had been maintained at the laboratory conditions of at 70% relative humidity for almost 150 generations, were subjected to cytogenetical analyses. the ‘instant’ landmarks in the squash preparations of the salivary gland chromosomes are indicative of cytotaxonomic characteristics of the species. the characteri...

The objective of this study was to design DNA probe sets that enable the detection of chromosome aberrations in acute myeloid leukemia (AML) by interphase cytogenetics using fluorescence in situ hybridization (FISH) and to compare the results of interphase cytogenetics with those of conventional chromosome banding analysis. One hundred five consecutive patients with adult AML entered on a multi...

the short arm of rye 1r chromosome (1rs) carries genes for resistance to diseases and pests. the presence of the 1rs arm also enhance adaptation, stress tolerance and yield potential of bread wheat. in the present study 17 bread wheat cultivars were screened to identify 1bl.1rs translocation (t1bl.1rs) by isoelectric focusing, c-banding and fluorescence in situ hybridization (fish) methods. iso...

ABSTRACT: Cytological review of 59 aeshnid species and cytogenetic investigations on Anax ephippiger, immaculifrons, indicus, nigrofasciatus nigrolineatus, parthenope, Gynacantha subinterrupta the family Aeshnidae by carbol fuchsin staining C - banding have been under taken. All posses 2n = 27m with X0 XX sex determination except ephippiger 14 + neo XY, resulted 13 simultaneous fusions among au...

We investigated the karyotype of 18 didelphid species captured at 13 localities in the Brazilian Amazon, after conventional staining, C-banding, Ag-NOR and fluorescent in situ hybridization (FISH) using the 18S rDNA probe. Variations were found in the X chromosome, heterochromatin distribution and the 18S rDNA sequence. The main variation observed was in the position of the centromere in the X ...

Conventional alkaline C-banding was found to be an effective additional step in restriction endonuclease banding to improve staining contrast and to reveal hidden changes in the heterochromatic blocks. Examples of these cases are demonstrated on the bovine chromosome set using Hinf I and Taq I endonucleases in the digestion experiments.

We performed a cytogenetic study on 140 nonpolymalformed patients with mental retardation of clinically undefined origin, using a high resolution banding technique, to determine how much chromosome abnormalities contribute to the etiology of this condition. A total of 15 patients (10.7%) were found to have autosomal or sex chromosomal abnormalities. Autosomal abnormalities included partial mono...