BACKGROUND The lack of well standardized or characterized extracts that contain the relevant allergens of the appropriate fungus is resulting in a high heterogeneity of the commercial preparation. MATERIAL AND METHODS Immunochemical detection of the allergens composition of spore and mycelium of C. cladosporioides was studied by electroblotting using sera from Cladosporium allergic patients a...

depends on numerous factors, e.g. suitable growth substrate, biorhythms of the species, biotic and abiotic factors that influence the release, dispersion, and deposition of fungal propagules (1). Members of Cladosporium, Alternaria, Penicillium and Aspergillus genera were permanently present in many aeromycological surveys of outdoor and indoor environments. In general, in temperate climate, in...

In addition to the known mutisicoumaranones A (1) and B (2), the methanolic extract of the aerial parts of the shrub Mutisia friesiana afforded two new 5-methylcoumaranones, mutisicoumaranones C (3) and D (4). Their structures were elucidated by spectroscopic methods. (13)C NMR data for mutisicoumaranones A and B are reported for the first time. All compounds showed antifungal activity against ...

The fungi on Meju are known to play an important role as degrader of macromolecule of soybeans. In order to elucidate the origin of fungi on traditional Meju, mycobiota of the air both inside and outside traditional Meju fermentation rooms was examined. From 11 samples of air collected from inside and outside of 7 Meju fermentation rooms, 37 genera and 90 species of fungi were identified. In ou...

Mold specific quantitative PCR (MSQPCR) was used to measure the concentrations of the 36 mold species in indoor and outdoor air samples that were taken simultaneously for 48 h in and around 17 homes in Cincinnati, Ohio. The total spore concentrations of 353 per m(3) of indoor air and 827 per m(3) of outdoor air samples were significantly different (p<or=0.05). However, only the concentrations o...

The addition of an HDAC inhibitor, suberoylanilide hydroxamic acid (SBHA), to the culture medium of Cladosporium colocasiae, dramatically altered its metabolic profiles. Analysis of the culture broth extract led to the isolation of two new acetylenic sterols (1-2). The isolated compounds were further evaluated for their cytotoxic and antibacterial activities. Compound 1 showed activity against ...