mastitis is one of the most important diseases of dairy cattle in the world. the identification and characterization of the constituent proteins in milk can be useful for studying the biochemistry and pathogenesis of mastitis. in this study, the electrophoretic patterns of milk from 10 healthy and 30 mastitic cows were studied. all of the latter milk samples were california mastitis test (cmt) ...

Metabolic disorders in animals are one of the most important problems in modern cattle production. The paper presents results characterising the functional state of the liver in dairy cows with clinical ketosis. The study was conducted on 17 Holstein cows (9 of them were clinically healthy with negative test for ketone bodies in urine and 8 with positive test) in their second to fourth lactatio...

To obtain blood biochemical basic data of Japanese Black breeding cows in Kagoshima Prefecture, Japan, blood samples were obtained from 857 clinically healthy multiparous cows on 71 farms. Cows were divided into three stages: the prepartum stage (between 20 and 80 days before parturition, n=290), lactation stage (between 20 and 80 days after parturition, n=283) and maintenance stage (between 12...

BACKGROUND Downer cow syndrome (DCS) is often diagnosed in dairy cattle during the early post-partum period. The etiology of this condition is not completely understood, as it can be related to the energetic or electrolyte metabolism, as well as to infectious diseases or to trauma. HYPOTHESIS/OBJECTIVES The aim of this study is to compare energy metabolism and insulin sensitivity indices and ...

The aim of the present study was to investigate inline lactate dehydrogenase (LDH) dynamic changes based on different cow factors - number and stages lactation, milk yield, status reproduction in clinically healthy dairy cows. In Herd Navigator system, LDH activity levels (μmol/min per litre) were measured using dry-stick technology. A total 378 cows selected. According their reproductive statu...

Postpartum endometritis compromises milk production and fertility in high-producing dairy cows. Infection of the endometrium induces an inflammatory response with secretion of cytokines that lead to polymorphonuclear cells (PMN) influx and bacterial clearance. Considering that only a portion of cows with endometritis is eligible for clinical diagnosis, there is an increasing effort for developi...

To obtain blood biochemical basic data of Japanese Black breeding cows in Kagoshima Prefecture, Japan, blood samples were obtained from 857 clinically healthy multiparous cows on 71 farms. Cows were divided into three stages: the prepartum stage (between 20 and 80 days before parturition, n=290), lactation stage (between 20 and 80 days after parturition, n=283) and maintenance stage (between 12...

CD40 and CD40 ligand (CD40L) have costimulatory effects as part of a complex series of events in host immunity. In this study, the expression of CD40 and CD40L on peripheral blood mononuclear cells (PBMCs) isolated from cattle with Johne's disease were measured on freshly isolated PBMCs and on cells cultured for 8, 24, and 72 h in the presence or absence of live Mycobacterium avium subsp. parat...

Ketosis is a common metabolic disorder frequently observed in dairy cows during the early lactation period. The objective of this study was to examine the changes in β-hydroxybutyrate (BHBA), glucose, non-esterified fatty acids (NEFA), glutathion peroxidase (GSH-Px), superoxide dismutase (SOD), xanthine oxidase (XOD), malonaldehyde, catalase and total antioxidant capacity (T-AOC) concentrations...

BACKGROUND Intravenous regional anaesthesia (IVRA) and hindfoot four-point nerve block anaesthesia (NBA) are recommended for local anaesthesia (LA) in the distal limb of dairy cows. Two studies were conducted to compare the efficacy, time until onset and stress responses to IVRA and NBA in dairy cows. In the first cross-over designed study, eight healthy unsedated German Holstein cows, restrain...