نتایج جستجو برای: clonogenic assay

تعداد نتایج: 224283  

Journal: :Blood 1995
I A Haagen A J Geerars W B de Lau B J Bast B C de Gast

To evaluate the potency by which human T cells are targeted and activated by bispecific monoclonal antibodies (BsAbs) to lyse tumor cells, a clonogenic assay was developed. The efficacy of a CD3 x CD19 BsAb binding to both the CD3 T-cell antigen and the CD19 B-cell antigen was already proven in 51Cr-release assays and in 3-day activation cultures. To achieve more quantitative results, a 14-day ...

2012
Karl Buch Tanja Peters Thomas Nawroth Markus Sänger Heinz Schmidberger Peter Langguth

For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be performed with a large number of samples in short time using multi-well-plat...

Journal: :Future Journal of Pharmaceutical Sciences 2022

Abstract Background Medicinal herbs in Malaysia like Clinacanthus nutans (CN) traditionally are used the treatment of various diseases and cancers. The present research was conducted to determine effects C. leaf different solvent extracts on human breast cancer cell lines (MCF-7). antiproliferative growth survival dichloromethane CN (CNDCM), as well short- long-term through metastasis, apoptosi...

Introduction: Thyroid cancer is the most common endocrine malignancy tumor, and its incidence is increasing. Chemotherapy drugs such as Paclitaxel (PTX) are common treatment for cancer, but they have many side effects. Plants are as a source of anticancer agents.  The purpose of this study was the assessment of cytotoxic effects of hydro-alcoholic extract in comparison with those of Paclitaxel ...

Journal: :Investigative ophthalmology & visual science 2010
Lan Huang Matthew Harkenrider Meredith Thompson Pingyu Zeng Hiromi Tanaka David Gilley David A Ingram Joseph A Bonanno Mervin C Yoder

PURPOSE To test the hypothesis that the robust expansion of bovine corneal endothelial cells (BCECs) in vitro is due to the presence of individual endothelial cells with various levels of proliferative potential. METHODS BCECs and bovine vascular endothelial cells (ECs) derived from aorta, coronary artery, and pulmonary artery were cultivated in optimized medium. These cell populations were c...

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