To evaluate the value of IgG avidity in diagnosis of congenital cytomegalovirus (CMV) infection in newborns and infants we collected serum samples from 40 infants under 12 months of age with suspected congenital CMV infection. Sera were tested for IgM, IgG and IgG avidity. For 25 of them, virus isolation and/or polymerase chain reaction (PCR) on urine specimens were performed. Thirteen (32.5%) ...

To determine if cytomegalovirus (CMV) infection is a risk factor for primary coronary artery disease (CAD), the association between CMV infection and CAD (>50% blockage in any coronary artery) was investigated in nearly 900 successive nontransplant patients undergoing coronary angiography. By use of logistic regression, older age (P <.001), white race (P <.001), gender (P <.001), hypercholester...

Interferon (IFN) was produced after exposure of human mononuclear leukocytes and bone marrow cells to infectious or noninfectious cytomegalovirus (CMV) in vitro. The IFN was generated mainly by non-B lymphocytes. Both alpha- and gamma-type IFN could be demonstrated. CMV antigens were usually not demonstrable in CMV-exposed leukocytes. Addition of anti-IFN antibodies did not induce CMV antigens....

BACKGROUND Cytomegalovirus (CMV) infection in susceptible patients is associated with serious morbidity and a high mortality. Transmission of cytomegalovirus infection through blood transfusion is markedly reduced by transfusion of CMV seronegative blood products, or by transfusion of leucodepleted blood products. OBJECTIVE To determine the prevalence CMV IgG and IgM antibodies among blood do...

Cytomegalovirus (CMV) infection exerts an enormous effect on human immunity, as it is associated with an immune-impaired response, a variety of chronic diseases, and overall survival in elderly individuals. Levels of anti-CMV antibodies may be associated with the differentiation degree of T cell subsets. Titers are significantly higher in the elderly and positively correlated with specific CD4(...

BACKGROUND Cytomegalovirus (CMV) is the most common infectious cause of mental disability in newborns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. METHODS An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a bios...

Background: We analyzed both early and late (persistent) phases of each cytomegalovirus (CMV) antibody in mothers with primary CMV infection during pregnancy subsequent congenital for a long period from to after delivery using our stored serum samples. Methods: used samples obtained immunoglobulin (Ig) G seroconversion infant infection. antibodies, including IgG titer, IgM avidity, were assesse...

OBJECTIVE Previous histochemical studies in the demyelinating form of Guillain-Barré syndrome (GBS), acute inflammatory demyelinating polyneuropathy (AIDP), have shown complement deposition on the surface of Schwann cells, and therefore unknown epitopes would be present on the outer surface of Schwann cells. METHODS We used a proteomic-based approach to search for the target molecules of AIDP...

The results of studies reported previously (1-3) demonstrated heterogeneity in the neutralizing properties of early (7-day) and late (hyperimmune) 7S and 19S antibodies prepared in rabbits against human herpes simplex virus (HSV) and the simian herpesvirus SA8. In the case of the late antibodies, we showed reciprocal cross-neutralization with the 7S antibodies, whereas neutralization by the 19S...

BACKGROUND Although neutralizing antibodies play a central role in the control of cytomegalovirus (CMV) dissemination, little is known about the response of B lymphocytes to primary human CMV infection. METHODS The proportion, phenotype, specificity, and functionality of B-cell subsets were studied in a cohort of pregnant women with primary CMV infection. CMV-seronegative pregnant women, as w...