Neurons are multifaceted cells that carry information essential for a variety of functions including sensation, motor movement, learning, and memory. Studying neurons in vivo can be challenging due to their complexity, their varied and dynamic environments, and technical limitations. For these reasons, studying neurons in vitro can prove beneficial to unravel the complex mysteries of neurons. T...

background: the present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. materials and methods: sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. isolated spermatogonial cells were co-cultured with sertoli cells in minimal essential medi...

This study investigated the cognition of 70 Tunisian teachers on the place of culture in English education. It showed that Tunisian teachers believe that English textbooks and curricular documents are not specific about the cultural dimension of EFL. It also revealed that L2 teachers, whose mother culture is distant from that associated with L2, hold ambivalent attitudes towards culture. They a...

In order to examine how implanted bone marrow stromal cells (BMSCs) encourage peripheral nerve regeneration, the present study investigated the interaction of BMSCs and Schwann cells (SCs) using an indirect in vitro co‑culture model. SCs and BMSCs were obtained from adult Sprague‑Dawley rats. The passaged BMSCs were CD29‑ and CD44‑positive but CD45‑negative and were co‑cultured with the primary...

introduction: in this study we examined the effect of ampullary and isthmic epithelial cell of golden hamster oviduct on n-mary mouse embryo development during 4-days in vitro co - culture, and the effect of injected gonadotropins on the quality of co-culture. materials and methods: golden hamster oviducts were isolated 18-24h after treating the hamster with hcg. they were flushed with dmem / h...

objective: to study the effects of different matrices and co-culture systems on cultured limbal stem cells (lscs). materials and methods: limbal explants were co-cultured with limbal fibroblasts (lf) and/or mouse embryonic fibroblasts (mef) on filter inserts coated with amniotic membrane (am), matrigel (mat) and collagen type i (col). results: this study revealed that am facilitated the cell mi...

The formation, maintenance, and repair of biological tissues rely on the interaction of cells with other cell types and with their extracellular matrix [2, 3]. These cellular communications can be bi-directional or multi-dimensional and may occur at both macroand micro-scales. Depending on the hypothesis of interest, co-culture models are used to discern the individual and collective effects of...

introduction: the suspected water samples contaminated with legionella were enriched with acanthamoebae. small numbers of bacteria (not detectable by culture) can be multiplied in amoebae up to culturable level in co-culture condition. the conventional culture method for legionnaires' disease bacterium always suffers from low sensitivity. this co-culture method can significantly improve culture...

OBJECTIVES This study aims to explore whether Huperzine A (HupA) could protect neural stem cells against amyloid beta-peptide Aβ induced apoptosis in a neural stem cells (NSCs) and microglia co-culture system. METHODS Rat NSCs and microglial cells were isolated, cultured and identified with immunofluorescence Assays (IFA). Co-culture systems of NSCs and microglial cells were employed using Tr...