In Escherichia coli, the cold shock response is exerted upon a temperature change from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including polynucleotide phosphorylase (PNPase), during acclimation phase. In E. coli, PNPase is essential for growth at low temperatures; however, its exact role in this essential function has not been fully elucid...

The 70-kD heat-shock proteins (HSP70s) are encoded by a multigene family in eukaryotes. In plants, the 70-kD heat-shock cognate (HSC70) proteins are located in organellar and cytosolic compartments of cells in most tissues. Previous work has indicated that HSC70 proteins of spinach (Spinacia oleracea) are actively synthesized during cold-acclimating conditions. We have isolated, sequenced, and ...

CspA, the major cold-shock protein of Escherichia coli, is dramatically induced during the cold-shock response. The amino acid sequence of CspA shows 43% identity to the "cold-shock domain" of the eukaryotic Y-box protein family, which interacts with RNA and DNA to regulate their functions. Here, we demonstrate that CspA binds to RNA as a chaperone. First, CspA cooperatively binds to heat-denat...

Patients in surgical shock present clinical evidence of a diminished peripheral circulation. The extremities are cold, the pulse is feeble, and the veins are empty of blood. An increase in the peripheral vascular resistance in experimental shock has been demonstrated by Erlanger, Gesell and Gasser (1). Quantitative measurements, however, on the reduction in circulation in human cases of shock h...

Jets are observed to stir up multi-phase turbulence in the inter-stellar medium as well as far beyond the host galaxy. Here we present detailed simulations of this process. We evolve the hydrodynamics equations with optically thin cooling for a 3D Kelvin Helmholtz setup with one initial cold cloud. The cloud is quickly disrupted, but the fragments remain cold and are spread throughout our simul...

In vivo protein expression in the abdominal viscera of C. v. slmorensis was examined from aduh flies that were cold shocked for various lengths of time at O, 10, or 15°C and labelled at 25°C with 35S-methionine at 0, 2, 4 and 6 hr during the recovery period. In vitro labelling showed that seven unique proteins (23, 40, 43, 48, 60, 70 and 92 kDa) were produced in C. v. sonorensis exposed to low ...

For many years, non-freezing cold shock injury has been associated with damage to the cell membrane. In this study, we enhanced membrane cholesterol levels of Drosophila melanogaster by raising larvae on a cholesterol-augmented diet. Diet augmentation significantly increased the amount of cholesterol in the cell membranes of the adult flies (1.57+/-0.17 nmol per mg vs. 0.93+/-0.11 nmol per mg)....

Colonization of the human nasopharynx exposes Moraxella catarrhalis, a common cause of otitis media in children and exacerbations of chronic obstructive pulmonary disease in adults, to sudden downshifts in temperature, occurring when the host breathes cold air. We investigated whether in vitro cold shock influences the expressions of the outer membrane adhesins UspA1 and hemagglutinin, which ar...