Early detection and identification of pathogenic bacteria such as Escherichia coli (E. coli) is an essential task for public health. The conventional culture-based methods bacterial colony usually take >24 hours to get the final read-out. Here, we demonstrate a colony-forming-unit (CFU) system exploiting thin-film-transistor (TFT)-based image sensor array that saves ~12 compared Environmental P...

MALLIGO, JOHN E. (Fort Detrick, Frederick, Md.). Evaluation of an automatic electronic device for counting bacterial colonies. Appl. Microbiol. 13:931-934. 1965.-An automatic colony counter was tested extensively with colonies of two bacterial species, Serratia marcescens and Bacillus subtilis var. niger, grown on agar media. A stable relationship was established between machine counts and coun...

BACKGROUND Information about the long-term follow-up and safety of granulocyte colony-stimulating factor administration to healthy donors is limited. The aims of this study were to analyze the side effects of granulocyte colony-stimulating factor administration in donors included in a Spanish Registry of hematopoietic stem cell donors and to determine the long-term outcome of these donors. DE...

The study assess some herbal agents used for fumigations as listed in Ayurvedic literature for various applications. Here coarse powder of 19 freshly collected and dried herbal agents were used for fumigating a room of about 594 cu .ft. volume. Air samples were collected using Nutrient Agar plates just before , 15 minutes and 45 minutes after fumigation from both ground and top. Assessment of t...

Primitive hematopoietic cells released into the peripheral blood (PB) were studied in 50 patients with high-grade non-Hodgkin's lymphoma enrolled in a phase III trial of intensive weekly chemotherapy (VAPEC-B) alone or with granulocyte colony-stimulating factor (G-CSF). Mononuclear cells numbers were monitored and their in vitro growth potential assessed in clonogenic progenitor cell assays and...

RATIONALE Repair of damaged endothelium is important in recovery from acute lung injury. In animal models, bone marrow-derived endothelial progenitor cells differentiate into mature endothelium and assist in repairing damaged vasculature. OBJECTIVES The quantity of endothelial progenitor cells in patients with acute lung injury is unknown. We hypothesize that increased numbers of circulating ...

Protein kinase C (PKC) is a Ca++- and phospholipid-dependent protein kinase activated by diacylglycerol that is either released from cell membranes in response to certain growth factors or mimicked by 12-O-tetradecanoyl phorbol-13-acetate (TPA). We studied the effects of TPA on interleukin-3 (IL-3)-dependent colony formation of mouse bone marrow cells from mice injected with 5-fluorouracil 2 da...