The colony-forming potential of peripheral blood cells from ten patients with acute lymphocytic leukemia, as well as their urinary colony-stimulating activity levels, has been studied. Peripheral white blood cells from 4 of 10 patients with acute lymphocytic leukemia formed significant numbers of colonies when grown on feeder layers of normal human white blood cells. The colonies were composed ...

Injection of testosterone, nortestosterone, or oxymetholone markedly enhanced granulopoietic recovery in mice made neutropenic by l,3-bis(2-chloroethyl)-l-nitrosourea, vinblastine, or cyclophosphamide. Blood neutrophils rose to six times normal counts, and the bone marrow showed sequential increases first in dividing granulocytic elements and later in the nondividing reserve. The steroid-induce...

Heat-treated breastmilk is one infant-feeding option recommended by the WHO to reduce mother-to-child transmission of HIV in developing countries. Flash-heat, a simple pasteurization method that a mother could perform in her home, has been shown to inactivate cell-free HIV-1. Since heating may affect the naturally occurring antimicrobial properties found in breastmilk, storing heated breastmilk...

Although all of 14 clinical isolates of Haemophilus influenzae type b strains demonstrated rifampin susceptibility in vitro (minimal inhibitory concentration less than or equal to 0.4 microgram/ml) when an inoculum of 10(4) colony-forming units (CFU) was used, 10 of the 14 strains manifested resistance to this agent when an inoculum of 10(8) CFU was tested. The mutation rate for rifampin resist...

BACKGROUND Non-touch fittings have been reported to be susceptible for Pseudomonas aeruginosa accumulation. A number of factors may contribute to this, including the frequency of usage, duration of water stagnation, or presence of plastic materials. Programmable non-touch fittings are appearing which allow regular automated post-flushing with cold water to prevent water stagnation. However, the...

Injection of testosterone, nortestosterone, or oxymetholone markedly enhanced granulopoietic recovery in mice made neutropenic by l,3-bis(2-chloroethyl)-l-nitrosourea, vinblastine, or cyclophosphamide. Blood neutrophils rose to six times normal counts, and the bone marrow showed sequential increases first in dividing granulocytic elements and later in the nondividing reserve. The steroid-induce...

Further in vitro studies of hematopoietic regulation were carried out in two patients with polycythemia vera who were also heterozygotes (Gd(B)/Gd(A)) for glucose-6-phosphate-dehydrogenase (G-6-PD). While only G-6-PD type A was detectable in circulating erythrocytes, granulocytes and platelets, cultures of peripheral blood and marrow from one patient revealed a substantial number of G-6-PD type...

The aim of this study was to model the growth, in a model system, of toxigenic strains of Bacillus cereus as a function of temperature, pH and water activity. Optical density (OD) values were transformed into numbers of colony-forming units (CFU) by the use of a 'calibrating' relation. The growth curves were then fitted to the Gompertz function which allowed the estimation of the lag-time (lamb...

The effects of mononuclear cells from nontransfused and transfused dogs on the growth of erythroid colonies (EC) from DLA-identical littermates were studied. To this purpose, marrow samples were cultured both before and after one to three transfusions from the DLA-identical littermate. Mononuclear cells obtained before transfusions and cocultured with autologous marrow or marrow from DLAidentic...

Purified natural murine I cell (macrophage) colony-stimulating factor (nCSF-1 ) and purified recombinant murine interleukin-3 (rlL-3) were administered to normal or lactoferrin-pretreated mice 20 to 24 hours before sacrifice. rlL-3 and nCSF-1 administered separately increased the percentage of macrophage high-proliferative potential colonyforming cells (HPP-CFC) and low-proliferative potential ...