This study describes the isolation, serotyping and genotyping of 54 infectious bronchitis virus (IBV) cases predominantly in KwaZulu-Natal and compared to several isolates from other South African provinces between 2011 and 2012 and several historic isolates. The results indicate the division of isolates into two different genotypes of IBV within the province, Massachusetts (Mass)-like and QX-l...

The level o protection conferred by commercial vaccines against the new variant Nebraska 95 isolate was evaluated. Complete protection was observed when the IBV Arkansas vaccine was given and when the Massachusetts-Connecticut vaccine was given at 1 and at 1 and 10 days of age. However, partial protection was observed when the Massachusetts-Connecticut vaccine was given only at 10 days of age. ...

Inactivated oil-adjuvanted vaccines for ND, IB, and IC serotypes A and C (OILVAX NB2AC) have been marketed from 1993. In the outdoors, various inoculation sites have been used in chickens because to make the inoculation procedure easier. We examined whether differences would be obtained in the antibody response according to the inoculation sites; the subcutaneous inoculation into the back of th...

Newcastle disease (ND) is one of the most important diseases that affect birds; the epizootic nature of the disease has caused severe economic losses in the poultry industry worldwide. In this experiment ND virus (NDV) was inactivated by two different chemicals binary ethylenimine (BEI) and formaldehyde. Formaldehyde was used at 0.1%, while BEI was used at concentrations of 1 to 4 mM. NDV inact...

newcastle disease (nd) is one of the most important diseases that affect birds; the epizootic nature of the disease has caused severe economic losses in the poultry industry worldwide. in this experiment nd virus (ndv) was inactivated by two different chemicals binary ethylenimine (bei) and formaldehyde. formaldehyde was used at 0.1%, while bei was used at concentrations of 1 to 4 mm. ndv inact...

Newcastle disease is a highly contagious and economically important disease of poultry. Low-virulence Newcastle disease virus (NDV) strains such as B1 and LaSota have been used as live vaccines, with a proven track record of safety and efficacy. However, these vaccines do not completely prevent infection or virus shedding. Therefore, there is a need to enhance the immunogenicity of these vaccin...

Commercial chickens with a high level of maternal antibodies for Newcastle disease were vaccinated when newly hatched with Queensland V4 or Ulster 2C Newcastle disease virus (NDV) strains by nebulization. The exposure time to a fine aerosol of vaccine produced with an ultrasonic nebulizer was 60 sec. The chickens were challenged oculonasally with virulent NDV strain Texas GB in weekly intervals...

Newcastle Disease (ND) is an acute, highly contagious viral disease in poultry. Despite the intensive vaccination programs to control Newcastle Disease Virus (NDV), the recent infection of NDV in Iran has led to severe morbidity and mortality in Chukar Partridges. The aim of this study was to investigate the ability of Inactivated Oil Adjuvant Vaccine (IOAV) following use of live vaccine to hav...

In several recent papers, two published last year in the Journal of Virology by DiNapoli et al. (3) and Ge et al. (4), an earlier study by Martinez-Sobrido et al. in 2006 (6), and a review by Bukreyev et al. in 2006 (1), Newcastle disease virus (NDV) is proposed as a potential vector in the development of a novel bior multivalent vaccine. We question whether those studies satisfactorily address...