Yersinia enterocolitica O:9 bears a smooth lipopolysaccharide (S-LPS) of Brucella sp. O-chain A+C/Y epitopic structure and is a cause of false-positive serological reactions (FPSR) in standard tests for cattle brucellosis. Brucella S-LPS, cross-reacting S-LPSs representing several O-chain epitope combinations, Brucella core lipid A epitopes (rough LPS), Brucella abortus S-LPS-derived polysaccha...

A study was performed in order to validate ELISAs for the differentiation of FMDV-infected cattle from those that had merely been vaccinated. Sets of sera from naive and from vaccinated animals as well as from cattle that had been vaccinated and subsequently infected were tested for antibodies to nonstructural proteins (NSPs) of FMDV in a commercial test, the Chekit FMD-3ABC bo-ov (Bommeli/Inte...

background and objectives: invasive and non-invasive techniques are used to diagnose h. pylori infection. some factors influence the choice of a diagnostic test, such as the sensitivity and specificity of the tests, the clinical circumstances and the cost-effectiveness of the testing strategy. the aim of this study was to reveal the relationship between different h. pylori infection diagnosis m...

A competitive immunoassay for S-adenosyl-L-homocysteine (SAH) has been used in the clinical test for homocysteine via an enzymatic conversion reaction. Since S-adenosyl-l-homocysteine is a relatively unstable compound, we have used peptide library phage display to select a new mimotope peptide that interacts with the anti-SAH antibody. By immobilizing the synthetic peptide on solid phase as a c...

A blocking enzyme-linked immunosorbent assay (B-ELISA), using two neutralizing monoclonal antibodies (MAbs), was established and compared with the virus neutralization test (VNT) for detecting specific peste-des-petits-ruminants virus (PPRV) antibody in caprine and ovine sera. This technique was developed because VNT, the only available specific serological test for PPRV and the cross-reactive ...

Antibodies(Abs) have been always a major place in diagnostic laboratories. Many techniques like Enzyme-Linked Immuno Sorbent Assays (ELISA), immunofluorescence, Ab-microarray platforms, immunoblots, X-ray crystallography require the Abs. ELISA is main test that used Abs for Foot and Mouth Disease (FMDV) serology. It needs polyclonal or monoclonal to detect FMDV antigen For this purpose, solid-p...

In this study 253 serum samples from cattle showing respiratory disorders were tested by confirmatory Western Immunoblotting test (IBT), complement fixation (CFT) and competitive enzyme linked immunosorbent assay (c-ELISA). Two (0.8%) out of found to be positive two doubtful CFT while 7 (2.8%) as 25 (9.9%) them c-ELISA. On the other hand, a core profile antigenic bands needed identified at 110,...

1 Human Xanthine Oxidoreductase Determination by a Competitive ELISA Maria Giulia Battelli and Silvia Musiani .............................................. 3 2 Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC Richard W. Browne and Donald Armstrong ..................................... 13 3 Determination of...

BACKGROUND Horsefly sting causes allergic reactions in human body. However, our knowledge on horsefly allergens remains poor. OBJECTIVES To identify the novel horsefly allergens and characterize their properties. METHODS A native allergen protein Tab y 1 (apyrase) was purified from the salivary glands of the horsefly Tabanus yao Macquart by gel filtration and ion exchange chromatography. It...