نتایج جستجو برای: complement fixation tests
تعداد نتایج: 452032 فیلتر نتایج به سال:
The literature concerning various serological reactions in malaria in man has been well reviewed (1). According tothis review, attempts to obtain a specific complement fixation reaction with sera from human beings infected by Plasmodium vivax or by Plasmodium falciparum have led in the past to inconclusive results because no sensitive, specific, and easily standardized antigen was available. Du...
In both the complement fixation test (CFT) and the serum agglutination test (SAT) titres were reproducible for the most part within a twofold range. They seldom exceeded these limits and never a fourfold range. Brucella abortus was successfully isolated in 86% of serologically positive cases and evidence is presented to confirm the use of the 30 International Units/ml level in the CFT as being ...
The aim of this study was the application of fluorescence polarisation assay (FPA) for the examination of bovine sera and comparison of the results of the assay with the results of Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), and ELISA. Six hundred thirty-five sera from cattle, including 300 sera from healthy animals, 32 sera from animals regarded as s...
An indirect hemagglutination (IHA) test and a complement fixation (CF) test were evaluated from test results on sera from 212 human melioidosis patients of which 119 were culturally proved cases. Significant antibody titers (IHA titers of 1:40 or greater and CF titers of 1:4 or greater) were demonstrated with either test in all except five patients. IHA and CF titers ranged as high as 1:20,480 ...
Although the neutralization test is, in general, satisfactory for immunologic studies with a diversity of viruses, it is cumbersome and expensive. The complement fixation test, in contrast, is simple and rapid and has been successfully applied to a number of viral diseases, especially for diagnostic purposes. In the case of herpes simplex virus infections, however, the literature concerning the...
A method is described by which complement fixation is detected with an enzyme linked immunosorbant assay (ELISA) technique. The method obviates the need for sensitised sheep red blood cells as an indicator of complement fixation and the titration of complement is not critical. The dose response curve has the advantage of being steep and the test result is read photometrically. As test serum and...
Parent bacteria and L-forms of some streptococci of group A, group D, and Streptococcus MG share common antigens as shown by cross-reactivity in growth inhibition, complement fixation, and Ouchterlony tests. Cross-reactivity between these streptococci or their L-forms with a staphylococcal L-form, or Mycoplasma pneumoniae, was found only rarely and only in low-titer complement fixation tests. R...
A micro complement fixation assay was devised to measure the type-specificity of anti-M antibody. Unabsorbed sera were from rabbits hyperimmunized with heat-killed streptococci and from humans with naturally occurring antibody or immunized with purified M protein vaccines. These sera fixed complement only in the presence of homologous M proteins (serotypes 1, 3, 6, 12, and 14). The complement f...
Complement fixation titers of 1:2 and 1:4 with coccidioidin are not always of diagnostic significance. The concurrent use of the complement fixation and immunodiffusion tests is an effective means for specific serologic diagnosis of coccidioidomycosis in patients with low levels of complement-fixing antibodies. In routine testing, it is recommended that only sera that are negative for complemen...
In the course of experiments dealing with the specificity of certain heterologous tumqr transplants it was necessary to compare the reactivity of antisera to guinea-pig, rabbit, human, and mouse tissues with antigens of the homologous and of heterologous species. Preliminary attempts to carry out the titrations by means of the ring precipitin test failed to yield satisfactory results, due chief...
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