To study the dependence of the forward flux of creatine kinase (CK) on its substrates and products we designed an acute normoxic model of steady-state depletion of phosphocreatine (PCr) and adenylate in the isovolumic acetate-perfused rat heart. Various concentrations of PCr and ATP were induced by prior perfusion with 2 deoxy-d-glucose in the presence of insulin. The apparent rate constant ( k...

The world of education is required to always adapt the current development, especially in terms technology. Prospective teachers as one important elements are expected be able have three knowledge, namely pedagogy, content, technology, and combinations which commonly called Technological Pedagogical Content Knowledge (TPACK) ready become professional 21st century. This research aimed examine in...

The effectiveness of a learning is determined by the combination various dimensions knowledge with technology carried out teachers. This study aims to analyse TPACKs’ skills chemistry education students FKIP Untan who will become future method employs in this descriptive. participants involved research are class 2019 and 2020. instrument used TPACK questionnaire which consist 7 indicators. resu...

This study aimed to clarify the effect of phosphorus fertilizer on senescence and yield Tartary buckwheat under low-nitrogen treatment. A two-year field experiment investigate characteristics was conducted (Qianku 5) four application rates, 0(CK), 40(LP), 80(MP), 120 kg·ha−1 (HP), in absence nitrogen Compared with CK, MP treatment increased plant height, node number main stem, branch root-morph...

In a quest to better understand hereditary human deafness we focus on the motor protein myosin VIIA (MyoVIIA), mutations in which underlie dysfunctions in auditory, vestibular and visual processes. Proposed MyoVIIA inner ear functions include tethering transduction channels, trafficking proteins and anchoring hair cell stereocilia by associating with adherens junctions. Fueled by the interest t...

BACKGROUND Triacylglycerols (TAGs) are the most abundant form of storage oil in plants. They consist of three fatty acid chains (usually C16 or C18) covalently linked to glycerol. SDP1 is a specific lipase for the first step of TAG catabolism in Arabidopsis seeds. Arabidopsis mutants deficient in SDP1 accumulate high levels of oils, probably due to blockage in TAG degradation. We applied this k...

In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>...

We have tested the hypothesis that decreased functioning of creatine kinase (CK) at sites of energy production and utilization may contribute to alterations in energy fluxes and calcium homeostasis in congestive heart failure (CHF). Heart failure was induced by aortic banding in 3-week-old rats. Myofilaments, sarcoplasmic reticulum (SR), mitochondrial functions, and CK compartmentation were stu...

In adult regenerating cardiomyocytes in culture, in contrast to fetal cells, mitochondrial creatine kinase (Mi-CK) was expressed. In the same cell, two populations of mitochondria, differing in shape, in distribution within the cell and in content of Mi-CK, could be distinguished. Immunofluorescence studies using antibodies against Mi-CK revealed a characteristic staining pattern for the two ty...