BACKGROUND Urine amino-terminal probrain natriuretic peptide (NT-proBNP) concentrations may exclude the presence of heart failure and provide insight into renal clearance mechanisms for human NT-proBNP. We characterized the molecular forms of urine NT-proBNP detected by immunoassay. METHODS Urine from patients with heart failure was subjected to HPLC and analyzed using immunoassays specific t...

of developing osteoporosis (2) and has been approved by the Food and Drug Administration for use in monitoring antiresorptive therapies. A more complete assessment of a patient's osteoporosis status requires, among other things, both bone mineral densitometry (a measure of current bone status) and measurement of a biochemical marker of bone turnover (a measure of the rate of change in bone stat...

OBJECTIVE Fasting is not routinely recommended for renal function tests, despite the known effects of cooked meat on creatinine. We therefore studied variation in creatinine and estimated glomerular filtration rate (eGFR) after a standardized cooked meat meal in 80 subjects: healthy volunteers and diabetic patients with chronic kidney disease (CKD) stages 1 and 2, 3a, 3b, and 4 (n = 16/group). ...

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

Gentamicin blood levels were monitored in 86 patients. Twenty-one patients had valley levels over 2 mug/ml and 36% of these patients developed abnormal serum creatinine or a further rise in creatinine. No patient had a rise in creatinine without a valley level over 2. The peak levels in patients with valleys over 2 were above 10 mug/ml in only one case, whereas four patients had peaks over 10 m...

SYNOPSIS The statement made in some standard textbooksthatBenedict'squalitativetestgivesa green, yellow, or orange-red precipitate with pure solutions of glucose of varying strength has been shown to be incorrect. Pure solutions ofglucose give only a bright red precipitate at all concentrations. These changes in the colour of the suspensions are observed with urinary glucose only. The differenc...

SYNOPSIS The statement made in some standard textbooksthatBenedict'squalitativetestgivesa green, yellow, or orange-red precipitate with pure solutions of glucose of varying strength has been shown to be incorrect. Pure solutions ofglucose give only a bright red precipitate at all concentrations. These changes in the colour of the suspensions are observed with urinary glucose only. The differenc...