OBJECTIVES Nitrosative stress plays an essential role in the pathogenesis of periodontal disease. The aim of this study is to analyze the gingival crevicular fluid and saliva nitrite and nitrate levels in periodontally healthy and diseased sites. MATERIAL AND METHODS A total of 60 individuals including, 20 chronic periodontitis and 20 gingivitis patients and 20 periodontally healthy controls ...

Four strains of oral treponemes and Treponema phagedenis Reiter synthesize and secrete phospholipase C (PLC), which was detected by the hydrolysis of p-nitrophenylphosphorylcholine. PLC was detected in gingival crevicular fluid from diseased but not from healthy sulci. The initiation and progression of periodontal lesions may begin with the hydrolysis of membrane phospholipids by PLC.

The concentrations of doxycycline and 4-de-dimethylaminotetracycline required to inhibit 50% of collagenase activity were found to be 15 to 30 microM for human neutrophil and gingival crevicular fluid collagenases. Fibroblast collagenase was relatively resistant to inhibition by tetracyclines; the 50% inhibitory concentrations of doxycycline and 4-de-dimethylaminotetracycline were 280 and 510 m...

Fabricating an esthetically pleasing removable partial denture while avoiding the unsightly display associated with conventional clasp assemblies presented a challenge to dentists. Among other techniques, this could be done using acetal resin clasps as simple and effective means of improving removable partial denture esthetics.1 Patients often cite lack of retention and poor esthetics as reason...

periodontal inflammation is associated with morphological changes in the blood vessels which may influence the regulation of gingival blood flow (gbf). our aim was to adapt the heat provocation test to the human gingiva to assess vascular reactivity in periodontal inflammation. method: gbf was recorded by laser doppler flowmetry before and after heat provocation in healthy volunteers (n = 50). ...

BACKGROUND This study provides a vital insight in assessing the clinical and biochemical changes in interleukin (IL)-1β levels in peri-miniscrew crevicular fluid (PMCF) during the course of orthodontic tooth movement. METHODS The study comprised the analysis of IL-1β in peri-miniscrew crevicular fluid obtained from crevices around the miniscrews inserted in 11 patients (eight females and thre...