A new type of interstrand DNA-DNA cross-link between abasic (Ap) sites and 2'-deoxyadenosine (dA) residues was recently reported, but the chemical structure and properties of this lesion were not rigorously established. Here we characterized the nucleoside cross-link remnant released by enzymatic digestion of duplex DNA containing the dA-Ap cross-link. A synthetic standard was prepared for the ...

background: empathy plays an important role in healthy dentist and patient relationship. hence, the aim of the study is to (a) to measure the self‑reported empathy levels among dental undergraduate and postgraduate students. (b) to review the trend of changes in empathy level with experience, age, and gender among dental undergraduate and postgraduate students. materials and methods: this cross...

A new type of interstrand cross-link resulting from the reaction of a DNA abasic site with a guanine residue on the opposing strand of the double helix was recently identified, but the chemical connectivity of the cross-link was not rigorously established. The work described here was designed to characterize the chemical structure and properties of dG-AP cross-links generated in duplex DNA. The...

The human genome is guarded against mutations by several DNA repair pathways. Common for all pathways, is the initiating event of detecting the DNA damage. This initial event is followed by the coordinated recruitment of various other DNA repair proteins needed for the repair. For instance, the MSH2/MSH6 complex detects DNA mismatches, while the MRN or KU70/KU80 complexes detect double strand b...

Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long DNAs caused by ICLs taking into account crosslinking effect in itself and con...