To clarify biaxial fracture properties of the unidirectional carbon-fiber-reinforced plastic (CFRP) composites, a cruciform specimen with two symmetric rectangular flat dents in thickness direction was developed, and tensile tests were carried out under 0º 90º loadings. The showed three modes, namely transverse crack (TC), fiber break (FB), mixture TC FB modes (TC&FB). According to strain measu...

This work deals with the identification of hyperelastic constitutive parameters using virtual fields method. The choice displacement is a crucial aspect method, typically for reducing sensitivity to measurement noise. A first and simple option generate randomly. Nevertheless, in case models which stress not linear function parameters, improving trivial an alternative strategy has be found. In p...

The binding of chromosomal protein HMG1 to a palindromic sequence that can form the cruciform structure in supercoiled DNA and the subsequent effect on the transcription of the sequence were examined with pBR322 and its derivative plasmids. The plasmid DNA under negative supercoiling showed a selective sensitivity to nuclease S1. HMG1 protected against the nuclease S1 digestion. The results of ...

An endonuclease specific for cruciform junctions has been purified from yeast cells treated with a DNA-damaging agent. The activity was followed through five chromatographic steps by assaying for the linearization of supercoiled plasmid DNA, which extrudes cruciform structures in vitro. The sites of cleavage on pColIR215 were sequenced, and nicks were located to positions symmetrically opposed ...

The necessity to increase performances in terms of lifetime and security in mechanical components or structures is the motivation for intense research in fatigue. Applications range from aeronautics to medical devices. With the development of new materials, there is no longer a fatigue limit in the classical sense, where it was accepted that the fatigue limit is the stress level such that there...

A DNA molecule under negative superhelical stress becomes susceptible to transitions to alternate structures. The accessible alternate conformations depend on base sequence and compete for occupancy. We have developed a method to calculate equilibrium distributions among the states available to such systems, as well as their average thermodynamic properties. Here we extend this approach to incl...

A cruciform-shaped oligo(phenylenevinylene) (OPV) with a phosphorescent rhenium(I) chromophore incorporated at the centre and grafted triphenylamines around the periphery was designed as a new organic photovoltaic material, in which the chelation of rhenium(I) to the cruciform successfully constructs a three-dimensional intramolecular charge transfer system and extends the exciton lifetime; as ...

There is an emerging consensus that secondary structures of DNA have the potential for genomic instability. Palindromic AT-rich repeats (PATRRs) are a characteristic sequence identified at each breakpoint of the recurrent constitutional t(11;22) and t(17;22) translocations in humans, named PATRR22 (approximately 600 bp), PATRR11 (approximately 450 bp) and PATRR17 (approximately 190 bp). The sec...

Plasmid pT181 DNA secondary structures have been analyzed in vitro by nuclease S1 digestion and in vivo by bromoacetaldehyde treatment. A cruciform structure occurring at the pT181 replication origin in vitro is greatly enhanced by the binding of the plasmid-encoded initiator protein RepC. In vivo a DNA secondary structure also existed in the replication origin. Its frequency of formation was c...

Two palindromic DNA sequences were analyzed with respect to their chemical reactivities with diethyl pyrocarbonate. In negatively supercoiled plasmid templates enhanced N7 carbethoxylation was found with individual purines located in presumptive single-stranded loops of DNA cruciform structures. No enhanced reactivity at these positions was observed in linear, relaxed or low superhelical densit...