Electron Cryo-microscopy (cryo-EM) is an important biophysical technique that produces 3dimensional (3D) images at different resolutions. De novo folding is becoming a promising approach to derive the backbone of proteins from the cryo-EM 3D images at medium resolutions. We summarize our findings in major steps of de novo folding and the challenges from the

Cryo-electron microscopy has seen incredible progress recently. From the first reconstruction of frozen hydrated samples to recent atomicresolution structures, structural biology is undergoing a revolution. However, until recently, the resolution of cryo-EM structures was often significantly lower than that of X-ray crystallography. We have reached a point where, for an increasing number of sam...

Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique that has experienced major advances in recent years. Technological developments in image recording as well as in processing software make it possible to obtain three-dimensional reconstructions of macromolecular assemblies at near-atomic resolution that were formerly obtained only by X-ray crystallograph...

BACKGROUND Transbronchial biopsy (TBB) which is performed with metal forceps (forceps TBB) has been accepted as a useful technique in establishing diagnoses of diffuse lung diseases (DLDs). The use of cryoprobes to obtain alveolar tissue (cryo-TBB) is a new method which is currently used by our institute as well as others with excellent results. OBJECTIVES To assess the safety of cryo-TBB com...

OBJECTIVES The aim of this study was to evaluate the efficacy and safety of catheter-based cryo-therapy for septal tachycardia substrates in pediatric patients. BACKGROUND Cryo-therapy may be particularly useful for ablation of septal tachycardias, including atrioventricular nodal re-entry tachycardia (AVNRT), atrioventricular reciprocating tachycardia (AVRT), and ventricular tachycardia (VT)...

Renal artery stenosis is increasing in prevalence. Angioplasty plus stenting has not proven to be better than medical management. There has been a reluctance to use available denervation methodologies in this condition. We studied conscious, chronically instrumented, two-kidney, one-clip (2K-1C) Goldblatt rats, a model of renovascular hypertension, to test the hypothesis that renal denervation ...

CONTEXT It is suggested that postinjury pain is difficult to examine; thus, investigators have developed experimental pain models. To minimize pain, cryotherapy (cryo) is applied, but reports on its effectiveness are limited. OBJECTIVE To investigate a pain model for the anterior knee and examine cryo in reducing the pain. DESIGN Controlled laboratory study. SETTING Therapeutic modality l...

Cryo-electron microscopy (cryo-EM) has become one of the main tools in structural biology over last decade. Nonetheless, details cryo-EM remain unknown to many researchers. microscopes are very expensive. Therefore, owning, maintaining, or renewing a instrument single laboratory university is often challenging. As been done for synchrotrons, it rational introduce shared facilities and allocate ...

The management of cane supply from growers’ fields to the mill involves many stakeholders and many tasks to be carried out along the chain, the successful combination of which impacts on the efficiency and profitability of the mill area. A simulation tool called MAGI has been developed to assist millers and growers in designing and assessing new ways of organising supply management. The issues ...

INTRODUCTION Extracellular vesicles (EV) are phospholipid bilayer-enclosed vesicles recognized as new mediators in intercellular communication and potential biomarkers of disease. They are found in many body fluids and mainly studied in fractions isolated from blood plasma in view of their potential in medicine. Due to the limitations of available analytical methods, morphological information o...