Though cryogenic storage is presumed to provide nearly infinite longevity to cells, the actual shelf life achieved under ultra-cold temperatures has not been addressed theoretically or empirically. Here, we report measurable changes in germination of dried seeds stored under liquid nitrogen conditions for >10 years. There was considerable variability in the extent of deterioration among species...

Cryogenic activity and stability of benzaldehyde lyase enzyme are achieved in lipidic mesophases, relying on the existence liquid water at subzero temperatures.

Abstract Detectors for direct dark matter search using noble gases in the liquid phase as a detection medium need to be coupled liquefaction, purification and recirculation systems. A dedicated cryogenic system has been assembled operated at INFN-Naples laboratory with aim liquefy purify argon used an active target detectors study scintillation ionization signals detected by large SiPM arrays. ...

Fluid replacement after hemorrhage usually results in hemodilution. Hemodilution leads to increased cerebral blood flow, which is known to be beneficial to the outcome of ischemic brain damage. However, the effect of hemodilution may be different in patients with head injuries and increased intracranial pressure (ICP). The aim of this study is to evaluate the effects of normovolemic hemodilutio...

The high speed steel is used as a cutting tool, since it has good quality and reliability at cheaper rate when compared to other cutting tools. So it is very important to increase the ability of the tool further for its reliability. So the best way is to increase the hardness. We have brainstormed to find out alternate tool materials. One novel method namely dipping the ordinary tool bit in a c...

deep cryogenic heat treatment is assumed as a supplementary heat treatment performed on steels before the finaltempering treatment to enhance the wear resistance and hardness of the steels. in this study, the effects of thedeep cryogenic heat treatment on the wear behavior and corrosion resistance of the 1.2080 tool steel were studiedusing the wear testing machine and polarization and impedance...

Cryogenic treatments and cryoprotective agents (CPAs) determine the survival rate of organisms that undergo cryopreservation, but their mechanisms of operation have not yet been characterised adequately. In particular, the way in which membrane lipids respond to cryogenic treatments and CPAs is unknown. We developed comparative profiles of the changes in membrane lipids among cryogenic treatmen...

Insulated pressure vessels are cryogenic-capable pressure vessels that can be fueled with liquid hydrogen (LH2) or ambient-temperature compressed hydrogen (CH2). Insulated pressure vessels offer the advantages of liquid hydrogen tanks (low weight and volume), with reduced disadvantages (fuel flexibility, lower energy requirement for hydrogen liquefaction and reduced evaporative losses). The wor...

The ponderomotive force, exerted upon all dielectric liquids by a non-uniform electric field, can be used for the remote, voltage-controlled manipulation of 10 to 100μL volumes of cryogenic liquids. This liquid dielectrophoretic (DEP) effect, imposed by specially designed electrodes, combines with capillarity to influence the hydrostatic equilibria of liquid deuterium. A simple, one-dimensional...