background: cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. the organism can be transmitted through water. since small number of c. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of c. parvum oocyst in samples with limited number of oocysts. methods: antibody was prepared against ...

We provide the first comprehensive analysis of any transcription factor family in Cryptosporidium, a basal-branching apicomplexan that is the second leading cause of infant diarrhea globally. AP2 domain-containing proteins have evolved to be the major regulatory family in the phylum to the exclusion of canonical regulators. We show that apicomplexan and perkinsid AP2 domains cluster distinctly ...

Cryptosporidium parvum is a protozoan parasite that is now recognized as one of the leading causes of diarrhea in young calves. To date, there are no drugs or preventive measures available for the control of this disease. We have developed an oral vaccine that, when given to calves at birth, protects against experimental challenge with C. parvum. However, when field tested on a large dairy oper...

Ureaplasma species, the most commonly isolated microorganisms in women with chorioamnionitis, are associated with preterm delivery. Chorioamnionitis increases the risk and severity of bronchopulmonary dysplasia and persistent pulmonary hypertension in newborns. It is not known whether the timing of exposure to inflammation in utero is an important contributor to the pathogenesis of bronchopulmo...

The first step in assessing the risk of water contamination by Cryptosporidium parvum oocysts from feedlot cattle (Bos taurus) production systems is to quantify the number of C. parvum oocysts present in the fecal material deposited by feedlot cattle. Our primary objective for this project was to estimate the daily environmental load of C. parvum oocysts in fecal material deposited by feedlot c...

Corynebacterium parvum has been administered i.p. to 14 patients with advanced ovarian cancer. Two patients had responded completely to cytoreductive surgery and combination chemotherapy prior to immunotherapy, and one patient with residual disease had received only a single course of C. parvum due to i.p. catheter malfunction. Among the 11 patients with residual disease evaluable for response,...

Tetracycline has been one of the most commonly used agents for producing a pleurodesis. However, it is no longer available due to more stringent requirements on the manufacturing process. The objective of this project was to determine whether Corynebacterium parvum is an effective sclerosant in an experimental model in rabbits. The following medications were instilled intrapleurally in anaesthe...

Infection of epithelial cells by Cryptosporidium parvum triggers a variety of host-cell innate and adaptive immune responses including release of cytokines/chemokines and up-regulation of antimicrobial peptides. The mechanisms that trigger these host-cell responses are unclear. Thus, we evaluated the role of TLRs in host-cell responses during C. parvum infection of cultured human biliary epithe...

INTRODUCTION Undernutrition is a recognized public health problem in Mexican children and cryptosporidiosis is probably a factor contributing to this problem. However, it remains a neglected and poorly attended infection in Mexico. This study aimed to determine the prevalence of Cryptosporidium parvum and to establish its association with the nutritional status in schoolchildren of northwestern...

New and improved methods are needed to disinfect pathogens in treated wastewater, especially highly resistant ones like Cryptosporidium parvum oocysts. In addition, new and improved methods are needed to detect pathogens and indicators for them in wastewater in order to monitor the efficacy of disinfection and other waste water treatment processes. Spores of the anaerobic bacterium Clostridium ...