Background & Aim: Cryptosporidium is zoonotic coccidian parasite that causes severe diarrhea in humans and animals worldwide. This study aimed to evaluate the soil contamination rate with Cryptosporidium oocysts of different public places in Tabriz, west of Iran. Methods: In this cross sectional study, 260 soil samples were collected by stratified sampling from 20 parks, 20 primary schools, 20...

An inoculum containing Cryptosporidium oocysts obtained from an adult human patient with acute transient diarrhoea induced enteritis in two newborn specific pathogen free lambs. Three days after inoculation the lambs became depressed and diarrhoeic, their milk intake was reduced and Cryptosporidium oocysts were detected in their faeces. Mucosal changes observed in the small intestine of one inf...

Epidemiologic and laboratory data suggest that coprodiagnostic methods may fail to detect Cryptosporidium oocysts in stool specimens of infected patients. To improve the efficacy of stool concentration procedures, we modified different steps of the Formalin-ethyl acetate (FEA) stool concentration technique and evaluated these modifications by examining stool samples seeded with known numbers of...

BACKGROUND As a waterborne pathogen, Cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. This study aimed to investigate the distribution of Cryptosporidium spp. in human and livestock wastewaters in Iran, by the 18S rRNA sequence analysis. METHODS A total of 54 raw wastewater samples collected from three urban treatment plants and two sl...

Preservation of Cryptosporidium oocysts in faecal specimens containing 75% ethanol is suitable for subsequent morphometric and molecular analysis. No significant morphologic alteration occurred after storage at ambient temperatures, ranging from 22 to 38 degrees C, for more than 2 years. After washing, sugar floatation and DNA extraction, a nested polymerase chain reaction targeting the small s...

Cryptosporidium parvum infectivity in a neonatal CD-1 mouse model was used to determine the dose needed to infect 50% of the population. The 50% infective dose was estimated to be 79 oocysts. It was observed that a mean oral inoculum of 23 oocysts produced infection in 2 of 25 neonatal mice 7 days postinoculation. All animals became infected when the mean oral dose exceeded 310 oocysts per anim...

Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.

Faecal samples from 291 dairy calves from 10 dairy herds in Kerman were examined to investigate theprevalence of Cryptosporidium infection. Cryptosporidium spp. oocysts were identified by using themodified Ziehl-Neelsen staining technique in 63 (21.65%) calves. Calves were grouped according to theirage as follows:

background: as a waterborne pathogen, cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. this study aimed to investigate the distribution of cryptosporidium spp. in human and livestock wastewaters in iran, by the 18s rrna sequence analysis. methods: a total of 54 raw wastewater samples collected from three urban treatment plants and two sl...