The trehalulose synthase (MutB) from Pseudomonas mesoacidophila MX-45, belonging to glycoside hydrolase family 13, catalyses the isomerization of sucrose to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as main products and glucose and fructose in residual amounts from the hydrolytic reaction. To date, a three-dime...

Autotaxin (ATX or ENPP2) is a secreted glycosylated mammalian enzyme that exhibits lysophospholipase D activity, hydrolyzing lysophosphatidylcholine to the signalling lipid lysophosphatidic acid. ATX is an approximately 100 kDa multi-domain protein encompassing two N-terminal somatomedin B-like domains, a central catalytic phosphodiesterase domain and a C-terminal nuclease-like domain. Protocol...

The purification procedure of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77 was improved by applying treatment with trypsin before chromatography. Purified protein samples both with and without trypsin treatment were successfully crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol as a precipitant. Both crystals belonged to space group P21, with unit-c...

It is shown that the addition of salt to liquid crystal cells, using a SiOx alignment layer, can actually reduce the ion concentration. This seeming contradiction may be explained by the ability of salt to complex with water and to aid the drying of the liquid crystal material. The results show a pathway to purifying liquid crystal devices to the extent needed for low-power low-refresh rate dis...

Objectives: The Phase transition scheme of Ferroelectric Liquid Crystal (FLC) sample has been studied in a temperature range 500C to 950C. Methods: material was procured from Merck, Germany and used as such without any further purification, for the measurement optical rotation transmittance. Polarizing Optical Microscopy (POM) technique different phases existing were observed by placing having ...

Cephamycin C is a β-lactam antibiotic used as a raw material in several commercial antibiotics. The production and purification process of this antibiotic requires a fast, simple and accurate method to quantify it. In this paper, it was developed a high-performance liquid chromatography method to determine cephamycin C concentration, in order to offer an option for the traditional, but laboriou...

In the present study, we continue investigation of the high-contrast vision in the inverted retina of the vertebrates eyes. We report a method of separation and purification of porcine (Sus scrofa domestica) intermediate filaments (IFs), extracted from the retinal Müller cells (MCs). We also report experimental and theoretical methods of measurements and calculations of the reduced resistivity ...

Adeno-associated virus (AAV) serotypes 1 to 5 are currently under development as clinical gene delivery vectors for the treatment of human diseases. However, the ubiquitous nature of their cell surface receptors, heparin sulfate (AAV2 and 3) and sialic acids (AAV4 and 5), can preclude specific tissue targeting in vivo. Structural studies of AAV4 were initiated to characterize its capsid surface...

the crystal structure of the title schiff base complex is obtained by single-crystal x-ray diffraction data.the solid state structure determination reveals that the coordination geometry around the copper(ii) center istetrahedrally distorted square-planar. the crystal packing shows one dimensional infinite chains which arisesfrom the intermolecular interaction and stabilize the crystal packing.

A recombinant form of Yersinia pestis YopM with a C-terminal polyhistidine affinity tag has been overproduced in Escherichia coli, purified to homogeneity and crystallized using the hanging-drop vapor-diffusion technique. Several different crystal forms were obtained. The most suitable crystals for X-ray diffraction belonged to space groups P4(2)22 (unit-cell parameters a = 109.36, b = 109.36, ...