BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

Gaillardia plants exhibiting symptoms characteristic of viral and phytoplasmal diseases were collected at botanical gardens and floriculture farms in Lithuania. Cucumber mosaic cucumovirus was isolated from diseased plants exhibiting symptoms characterized by stunting, flower breaking and malformation of petals. The virus was characterized based on electron microscopy, serology, reactions of in...

Peanut stunt virus-associated RNA 5 (PARNA 5), the satellite of a plant cucumovirus, is a linear RNA of 393 nucleotides with a 5' cap and a 3' hydroxyl group. Determination of its nucleotide sequence has revealed two consecutive open reading frames that together extend most of its length. Sequences at the 5' and 3' ends are homologous with those of the satellite of the related cucumber mosaic v...

Compared to other vegetable crops, the major viral constraints affecting pepper crops in the Mediterranean basin have been remarkably stable for the past 20 years. Among these viruses, the most prevalent ones are the seed-transmitted tobamoviruses; the aphid-transmitted Potato virus Y and Tobacco etch virus of the genus Potyvirus, and Cucumber mosaic virus member of the genus Cucumovirus; and t...

Cucumber plants exhibiting virus-like disease symptoms were collected in various gardens and greenhouses in Lithuania. Viral diseases were expressed by symptoms of mosaicmottling, yellow ringspots and vein necrosis of leaves. Growth and fruiting of plants were reduced. Results of the identification of viruses detected in cucumbers by the RT-PCR technique are presented here. Identification of ca...

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

In North Carolina, approximately 8% of the cucumber (Cucumis sativus) yield is lost to root-knot nematodes (Meloidogyne spp.) (Main and Gurtz, 1989). No resistance was found in cucumber after screening of 900 cultigens for resistance to Meloidogyne incognita race 3 (Walters, 1991). African horned cucumber (Cucumis metuliferus) has resistance to M. incognita (Fassuliotis, 1967; Walters, 1991), a...