Previous studies (3) of colony formation of 3T3, a cell line with the property of contact inhibition of replication, demonstrated a coincidence of cell contact and cessation of DNA synthesis and replication . Also, plating efficiency tests (3, 6) from monolayer cultures of 3T3 consistently have been in the range of 20%-30% for exponentially growing cells as well as cells in the state of contact...

BACKGROUND To investigate the antibiofilm efficacy of nitric oxide (NO) on soft contact lenses. METHODS Nitrite (NO precursor) release from various concentrations (0-1000 μM) of sodium nitrite (NaNO2, NO donor) was measured by Griess Assay. Cell viability assay was performed using human corneal epithelial cell under various concentration (0-1000 μM) of NaNO2. Biofilm formation on soft contact...

The evolution of pathological changes in the corneal epithelium may be studied by tissue culture methods. We have examined the effects of conventional hard contact lenses and hydrophilic soft lenses, in order to study the effect of the direct pressure of such lenses on the corneal epithelium and the effect of prolonged oxygen deprivation on cell growth. We have also observed the time of onset o...

Sublines of B7K21 hamster cells were derived by treatment with 5-fluoro-2 -deoxyuridine. These sublines demonstrated increased contact inhibition in vitro. The tumors resulting from injection of these sublines were examined, and cells from the tumors were returned to culture. Sublines with increased contact inhibition were less efficient at initiating tumors. All tumors, however, had an identic...

Indische culture is a that results from combination of European (Dutch) and local (Javanese) culture. Starting the arrival Dutch to Indonesia which resulted in cultural contact. Caused by this contact, then led acculturation between resulting new called This article was written with aim finding out about how can enter develop Indonesia. The method used literature study, namely collecting histor...

Non-culture diagnosis and serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. A serogroup B and C PCR ELISA assay for the non-culture diagnosis and serogroup determination has proved invaluable for enhanced epidemiological surveillance and contact management. A polymerase chain reaction assay, based on a restriction fragment l...

The mechanism of cell-cell contact dependent regulation of pericellular proteolysis in angiogenesis was examined by studying the expression of MMPs using isolated HUVECs in culture. Zymography, Immunoblot and RT-PCR analysis showed that the production and secretion of matrixmetalloproteinase-2 and matrixmetalloproteinase-9 by HUVECs in culture were high when they remain as individual cells and ...

We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we found that introducing cell-cell contact positively regulates proliferation, but that contact-mediat...