The response of cAMP to antidiuretic hormone (ADH) was studied using rat renal medullary cells in a monolayer culture. In addition, cAMP response to parathyroid hormone (PTH) was studied in renal cortical cells. As the culture aged, an increase in basal cAMP content and a gradual decrease in the cAMP responsiveness to arginine vasopressin (AVP) were observed. After 2 days of culture, AVP and hP...

Previously we observed that human adipose-derived stem cells (hADSCs) could form aggregation during culture in the presence of human serum (HS). In the present study, we have examined if the aggregation might result from the cell migration and analyzed the difference of cell adhesivity after culture in various conditions. When cells were cultured in fetal bovine serum (FBS) alone, there was no ...

The gapA gene encoding a novel RasGTPase-activating protein (RasGAP)-related protein was found to be disrupted in a cytokinesis mutant of Dictyostelium that grows as giant and multinucleate cells in a dish culture. The predicted sequence of the GAPA protein showed considerable homology to those of Gap1/Sar1 from fission yeast and the COOH-terminal half of mammalian IQGAPs, the similarity extend...

In typical in vitro studies on sonoporation, cells were cultured in a Petri dish or a multi-well plate filled with culture medium and sonicated by an ultrasound transducer placed under the dish or well. In this condition, ultrasound wave is reflected at the surface of the culture medium, and a complex standing wave field is generated inside the dish. Precise dosimetry is essential in ultrasound...

Mouse blastocysts in culture have been treated with increasing concentrations of cytosine arabinoside, bromodeoxyuridine or Colcemid. Concentrations of all three antimetabolites have been found which interfere with neither hatching of the blastocysts from their zona pellucidae nor subsequent attachment of the blastocysts to the culture dish, but which eventually result in death of the inner cel...

This is measured by a Boyden diffusion chamber. A filter separates cells, that can be labelled with radiochromium, and the attractant. Hence the number of neutrophils that move through the filter can be quantitated (Boyden, 1962; Ternowitz, 1985). One can also use a test in which there is migration of neutrophils under an agarose gel. Three wells are cut in agarose gel in a plastic tissue cultu...

A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass ...

Several techniques involving cell suspensions or cells attached to tissue culture dishes or cover slips have been described for measuring the flux of radioactively labeled substrates across the plasma membrane of eukaryotic cells (l-6). All these approaches are time consuming and cumbersome on a large scale. In this report we describe the construction of simple equipment based on the Costar 24-...